基于环介导等温扩增法的志贺氏菌和肠侵袭性大肠杆菌的灵敏快速检测
Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method.
作者信息
Song Tianyan, Toma Claudia, Nakasone Noboru, Iwanaga Masaaki
机构信息
Division of Bacterial Pathogenesis, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan.
出版信息
FEMS Microbiol Lett. 2005 Feb 1;243(1):259-63. doi: 10.1016/j.femsle.2004.12.014.
Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.
在此，我们报告一种用于检测志贺氏菌和侵袭性大肠杆菌的环介导等温扩增（LAMP）方法。该LAMP方法的靶标是这两种病原体均携带的ipaH基因。该LAMP方法能够在每个反应仅含少量细菌（8个菌落形成单位）的情况下，在2小时内高效检测到该基因。
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