[白细胞介素-10/血小板衍生生长因子/丝裂原活化蛋白激酶途径对肝星状细胞活化的调控]
[Regulation of hepatic stellate cell activation by interleukin-10/platelet derived growth factor/mitogen-activated protein kinase pathway].
作者信息
Li Tao, Leng Xi-Sheng, Qin Zhi-Zhong, Song Sheng-Han, Zhao Li, Xiong Liang-Fa, Peng Ji-Run
机构信息
Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing 100044, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2005 Jan;13(1):35-7.
OBJECTIVE
To investigate the regulatory effect of interleukin-10 (IL10) on the activation of hepatic stellate cells (HSC) through platelet derived growth factor (PDGF) and mitogen-activated protein kinase (MAPK) pathways.
METHODS
HSC were divided randomly into 4 groups. Group 1 served as a control. HSC were incubated with 1 ng/ml, 5 ng/ml, and 25 ng/ml IL-10 in groups 2, 3 and 4. RT-PCR and western blot were used to detect the expression of PDGF and MAPK protein ERK and p38 and alpha-SMA.
RESULTS
Compared with the control group, expressions of ERK, p38 and alpha-SMA of groups 2, 3 and 4 were significantly lower (F values were 240.47, 21.39, 28.86 respectively. IL-10 inhibited PDGF and MAPK protein ERK and p38 and alpha-SMA expression in a dose-dependent way.
CONCLUSION
IL-10 inhibits activation of HSC through the PDGF/MAPK pathway.
目的
通过血小板衍生生长因子（PDGF）和丝裂原活化蛋白激酶（MAPK）途径，研究白细胞介素-10（IL10）对肝星状细胞（HSC）激活的调节作用。
方法
将肝星状细胞随机分为4组。第1组作为对照组。第2、3和4组的肝星状细胞分别用1纳克/毫升、5纳克/毫升和25纳克/毫升的IL-10进行孵育。采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测PDGF、MAPK蛋白ERK和p38以及α-平滑肌肌动蛋白（α-SMA）的表达。
结果
与对照组相比，第2、3和4组的ERK、p38和α-SMA表达显著降低（F值分别为240.47、21.39、28.86）。IL-10以剂量依赖的方式抑制PDGF、MAPK蛋白ERK和p38以及α-SMA的表达。
结论
IL-10通过PDGF/MAPK途径抑制肝星状细胞的激活。
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