多嘧啶序列结合蛋白表达增加导致胰岛素mRNA水平升高。
Increased expression of polypyrimidine tract binding protein results in higher insulin mRNA levels.
作者信息
Fred Rikard G, Welsh Nils
机构信息
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
出版信息
Biochem Biophys Res Commun. 2005 Mar 4;328(1):38-42. doi: 10.1016/j.bbrc.2004.12.147.
The aim of this study was to further elucidate the role of the polypyrimidine tract binding protein (PTB) in the control of insulin mRNA stability. We observed that the glucose- or interleukin-1beta-induced increase in insulin mRNA was paralleled by an increase in PTB mRNA. To further test the hypothesis that PTB controls insulin gene expression, betaTC-6 cells were treated with a PTB-specific siRNA to modify the beta-cell content of PTB. Surprisingly, we observed an increase in PTB mRNA and PTB protein levels in response to the siRNA treatment. In addition, the PTB-siRNA treatment also increased insulin mRNA. We conclude that expression of the PTB gene controls insulin production.
本研究的目的是进一步阐明多嘧啶序列结合蛋白（PTB）在胰岛素mRNA稳定性调控中的作用。我们观察到，葡萄糖或白细胞介素-1β诱导的胰岛素mRNA增加与PTB mRNA的增加同时出现。为了进一步验证PTB控制胰岛素基因表达的假说，用PTB特异性小干扰RNA（siRNA）处理βTC-6细胞，以改变β细胞中PTB的含量。令人惊讶的是，我们观察到siRNA处理后PTB mRNA和PTB蛋白水平均增加。此外，PTB-siRNA处理也增加了胰岛素mRNA。我们得出结论，PTB基因的表达控制胰岛素的产生。
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