Fred Rikard G, Welsh Nils
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
Biochem Biophys Res Commun. 2005 Mar 4;328(1):38-42. doi: 10.1016/j.bbrc.2004.12.147.
The aim of this study was to further elucidate the role of the polypyrimidine tract binding protein (PTB) in the control of insulin mRNA stability. We observed that the glucose- or interleukin-1beta-induced increase in insulin mRNA was paralleled by an increase in PTB mRNA. To further test the hypothesis that PTB controls insulin gene expression, betaTC-6 cells were treated with a PTB-specific siRNA to modify the beta-cell content of PTB. Surprisingly, we observed an increase in PTB mRNA and PTB protein levels in response to the siRNA treatment. In addition, the PTB-siRNA treatment also increased insulin mRNA. We conclude that expression of the PTB gene controls insulin production.
本研究的目的是进一步阐明多嘧啶序列结合蛋白（PTB）在胰岛素mRNA稳定性调控中的作用。我们观察到，葡萄糖或白细胞介素-1β诱导的胰岛素mRNA增加与PTB mRNA的增加同时出现。为了进一步验证PTB控制胰岛素基因表达的假说，用PTB特异性小干扰RNA（siRNA）处理βTC-6细胞，以改变β细胞中PTB的含量。令人惊讶的是，我们观察到siRNA处理后PTB mRNA和PTB蛋白水平均增加。此外，PTB-siRNA处理也增加了胰岛素mRNA。我们得出结论，PTB基因的表达控制胰岛素的产生。
