Tripathi Manish K, Chaudhuri Gautam
Department of Microbiology, Meharry Medical College, Nashville, TN 37208, USA.
Biochem Biophys Res Commun. 2005 Mar 4;328(1):43-8. doi: 10.1016/j.bbrc.2004.12.142.
To understand the effects of the transient ablation of BRCA2 gene expression in dividing human breast cells, we transiently knocked down BRCA2 mRNA in HMEC and other cells. Microarray analysis of mRNAs revealed the down-regulation of the mRNAs of ubiquitin cross-reacting protein (UCRP) and the E2 enzyme that help conjugating UCRP to its target proteins, namely UBE2L6 (UbcH8), in BRCA2 ablated cells. UCRP is an interferon regulated protein, involved in cell growth and cell cycle events by participating in the degradation/modulation of cell cycle regulatory proteins. Quantitative-PCR and Northern analysis confirmed down-regulation of UCRP and UBE2L6 with BRCA2 knockdown, respectively. Since UCRP and UCRPylation have critical roles in the innate immunity against viral infection and during pregnancy, our observation may indicate new roles of the BRCA2 protein.
为了解BRCA2基因表达的瞬时消融对人乳腺分裂细胞的影响，我们在人乳腺上皮细胞（HMEC）和其他细胞中瞬时敲低BRCA2 mRNA。对mRNA的微阵列分析显示，在BRCA2消融的细胞中，泛素交叉反应蛋白（UCRP）以及有助于将UCRP与其靶蛋白结合的E2酶（即UBE2L6，UbcH8）的mRNA表达下调。UCRP是一种受干扰素调节的蛋白，通过参与细胞周期调节蛋白的降解/调节，参与细胞生长和细胞周期事件。定量PCR和Northern分析分别证实，BRCA2敲低后UCRP和UBE2L6表达下调。由于UCRP和UCRPylation在抗病毒感染的先天免疫以及妊娠期间起着关键作用，我们的观察结果可能表明BRCA2蛋白具有新的作用。
