Leavell Sarah, Wright Brittany, Scappino Lori, Sirriyah Jamal, Chen Chang, Clements John D, Burkhard Mary Jo
Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA.
Vaccine. 2005 Feb 10;23(12):1471-8. doi: 10.1016/j.vaccine.2004.09.017.
We examined the ability of FIV p24Gag to induce systemic and mucosal FIV-specific immune responses when delivered as a nasal immunogen alone, or with a mucosal adjuvant, Escherichia coli heat labile toxin LT(R192G). Nasal immunization with p24Gag alone induced FIV-specific immune responses but overall responses were weak, transient, and/or present only in a few animals. Co-administration of LT(R192G) resulted in strong FIV-specific serum IgG and enhanced salivary IgA responses. Moreover, FIV-specific IgA was detected in vaginal wash fluid from 6/6 cats co-immunized with LT(R192G) and p24Gag versus 1/6 immunized with p24Gag alone. This is the first report detailing induction of systemic or mucosal FIV-specific immune responses by nasal immunization alone. As such, this study demonstrates that nasal immunization of cats can be a relevant and effective route for the delivery of candidate vaccines. However, while nasal immunization of cats with p24Gag induces antigen-specific systemic immune responses, development of strong systemic and mucosal immune responses requires co-administration of a mucosal adjuvant, such as LT(R192G).
我们研究了猫免疫缺陷病毒（FIV）p24Gag单独作为鼻腔免疫原或与黏膜佐剂大肠杆菌不耐热毒素LT（R192G）一起给药时，诱导全身和黏膜FIV特异性免疫反应的能力。单独用p24Gag进行鼻腔免疫可诱导FIV特异性免疫反应，但总体反应较弱、短暂，且/或仅在少数动物中出现。联合给予LT（R192G）可产生强烈的FIV特异性血清IgG和增强的唾液IgA反应。此外，在与LT（R192G）和p24Gag联合免疫的6/6只猫的阴道冲洗液中检测到FIV特异性IgA，而单独用p24Gag免疫的猫中只有1/6检测到。这是第一份详细描述仅通过鼻腔免疫诱导全身或黏膜FIV特异性免疫反应的报告。因此，本研究表明，猫的鼻腔免疫可能是递送候选疫苗的一种相关且有效的途径。然而，虽然用p24Gag对猫进行鼻腔免疫可诱导抗原特异性全身免疫反应，但要产生强烈的全身和黏膜免疫反应需要联合给予黏膜佐剂，如LT（R192G）。
