Lindblom M
Biotechnol Bioeng. 1977 Feb;19(2):199-210. doi: 10.1002/bit.260190204.
The properties of intracellular RNase in disintegrated cell suspensions of Saccharomyces cerevisiae have been studied. The influence of salt addition and/or incubation of the suspension on the activity of RNase and on the degradation of endogenous RNA was determined. No significant change in the RNase activity in the disintegrated suspensions was obtained by addition of 3% NaCl or by incubation at 50 degrees C with 3% NaCl. During the incubation with NaCl the active RNase was able to degrade endogenous RNA. By incubation without salt the RNase was inactivated. Inactivation also occurred after extraction at alkaline pH. The RNase had an optima at pH 5-6 and temperatures between 50-60 degrees C. The main part of the RNase in the unincubated suspension was soluble also at pH 4.0. No serious protein degradation occurred during the short time incubation needed for RNA reduction. 70% of the protein in the suspensions was recovered in the precipitate at pH 4.0 after 20 min of incubation. The corresponding protein recovery from unincubated suspensions was 77%.
对酿酒酵母破碎细胞悬液中的细胞内核糖核酸酶（RNase）特性进行了研究。测定了添加盐和/或对悬液进行孵育对RNase活性以及内源性RNA降解的影响。添加3%氯化钠或在50℃下与3%氯化钠一起孵育，破碎悬液中的RNase活性均未出现显著变化。在与氯化钠孵育期间，活性RNase能够降解内源性RNA。在无盐条件下孵育，RNase会失活。在碱性pH值下提取后也会发生失活。RNase在pH 5 - 6以及50 - 60℃之间的温度下具有最佳活性。未孵育悬液中RNase的主要部分在pH 4.0时也是可溶的。在进行RNA减少所需的短时间孵育过程中，未发生严重的蛋白质降解。孵育20分钟后，pH 4.0时悬液中70%的蛋白质在沉淀中回收。未孵育悬液中相应的蛋白质回收率为77%。
