Sinha P R, Dutta S M
Folia Microbiol (Praha). 1984;29(4):337-42. doi: 10.1007/BF02875967.
Nuclease activities of the predominantly bacterial population obtained from buffalo rumen were investigated. Optimum temperature for hydrolysis of both DNA and RNA was 50 degrees C whereas DNAase activity was observed to be stable up to 50 degrees C, a decrease in RNAase activity was observed even after 40 degrees C. Two pH optima, one at 5.5 and the other at 7.5, were recorded for hydrolysis of DNA. RNAase activity was maximum between pH 6.0 to 7.0. Whereas DNAase activity was stable near its optimum pH, RNAase activity was stable between pH 7.0 to 8.5. Mn2+ ions stimulated DNAase activity. It was strongly inhibited by Hg2+, Zn2+, Pb2+ and Ag+. RNAase activity was stimulated by Mg2+ ions and was strongly inhibited by Hg2+, Cu2+, Zn2+ and Ag+. Cysteine hydrochloride and 2-mercaptoethanol stimulated DNAase activity. The activity was strongly inhibited by N-ethylmaleimide, 4-chloromercuribenzoate, 8-quinolinol, iodoacetic acid and 1,10-phenanthroline. RNAase activity was stimulated by cysteine hydrochloride, reduced glutathione and 2-mercaptoethanol and was strongly inhibited by 4-chloromercuribenzoate, 8-quinolinol and 2,2'bipyridyl.
对从水牛瘤胃中获得的主要细菌群体的核酸酶活性进行了研究。DNA和RNA水解的最佳温度均为50℃，而观察到DNA酶活性在50℃以下保持稳定，即使在40℃后RNA酶活性也出现下降。DNA水解记录到两个最佳pH值，一个在5.5，另一个在7.5。RNA酶活性在pH 6.0至7.0之间最高。虽然DNA酶活性在其最佳pH值附近稳定，但RNA酶活性在pH 7.0至8.5之间稳定。Mn2 +离子刺激DNA酶活性。它受到Hg2 +、Zn2 +、Pb2 +和Ag +的强烈抑制。Mg2 +离子刺激RNA酶活性，它受到Hg2 +、Cu2 +、Zn2 +和Ag +的强烈抑制。盐酸半胱氨酸和2-巯基乙醇刺激DNA酶活性。该活性受到N-乙基马来酰亚胺、4-氯汞苯甲酸、8-羟基喹啉、碘乙酸和1,10-菲咯啉的强烈抑制。RNA酶活性受到盐酸半胱氨酸、还原型谷胱甘肽和2-巯基乙醇的刺激，并受到4-氯汞苯甲酸、8-羟基喹啉和2,2'-联吡啶的强烈抑制。
