具有成纤维细胞生长因子-2和纤连蛋白片段的重组融合蛋白的工程构建与表达

Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin fragment.

作者信息

Jang Jun-Hyeog, Chung Chong-Pyoung

机构信息

Department of Biochemistry, Inha University College of Medicine, Jung Gu, Incheon 400-712, Korea.

出版信息

Biotechnol Lett. 2004 Dec;26(24):1837-40. doi: 10.1007/s10529-004-5278-1.

DOI:10.1007/s10529-004-5278-1

PMID:15672224

Abstract

There is a synergistic effect between fibronectin and fibroblast growth factor 2 (FGF-2) on osteoblast cell adhesion through an extracellular, signal-regulated kinase pathway. Here we describe the engineering of a fusion protein containing fibronectin fragment (FNIII9-10) connected to the COOH-terminus of FGF-2. Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone (p < 0.05).

摘要

纤连蛋白与成纤维细胞生长因子2（FGF-2）通过细胞外信号调节激酶途径对成骨细胞黏附具有协同作用。在此，我们描述了一种融合蛋白的构建，该融合蛋白包含连接到FGF-2羧基末端的纤连蛋白片段（FNIII9-10）。与单独的FNIII9-10相比，纯化的FGF2-FNIII9-10融合蛋白显著增加了MG63细胞的黏附和增殖（p < 0.05）。