小鼠和大鼠中MASP2基因座的组织及其表达谱
Organization of the MASP2 locus and its expression profile in mouse and rat.
作者信息
Stover Cordula M, Lynch Nicholas J, Hanson Steven J, Windbichler Michaela, Gregory Simon G, Schwaeble Wilhelm J
机构信息
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom.
出版信息
Mamm Genome. 2004 Nov;15(11):887-900. doi: 10.1007/s00335-004-3006-8.
The mouse, rat, and human MASP2 loci are situated on syntenic chromosome regions and are highly conserved. They comprise the genes for MASP-2/ MAp19, TAR DNA binding protein of 43 kDa, FRAP kinase, CDT6, Polymyositis-Scleroderma 100-kDa autoantigen, spermidine synthase, and TERE which were analyzed by annotation of available gene transcript data and cross-species comparison of available genomic sequences. The human and rat genes for spermidine synthase have an additional intron compared to the mouse gene. The mouse and rat genes for Polymyositis-Scleroderma 100-kDa autoantigen have an additional exon compared to the human gene. We find support for the hypothesis that the MAp19-specific exon within the MASP2 gene may have originated in a transposable element. Blocks of highly conserved intronic sequences were found in the MASP2 gene and the TARDBP gene. The expression of all genes within the MASP2 locus was analyzed in mouse and rat. The restricted expression of MASP-2 and MAp19 mRNA in liver contrasts with the ubiquitous expression of all neighboring genes studied.
小鼠、大鼠和人类的甘露聚糖结合凝集素相关丝氨酸蛋白酶2（MASP2）基因座位于同线染色体区域，且高度保守。它们包含MASP - 2/MAp19、43 kDa的TAR DNA结合蛋白、FRAP激酶、CDT6、多发性肌炎 - 硬皮病100 kDa自身抗原、亚精胺合酶和TERE的基因，这些基因通过对可用基因转录数据的注释和可用基因组序列的跨物种比较进行了分析。与小鼠基因相比，人类和大鼠的亚精胺合酶基因有一个额外的内含子。与人类基因相比，小鼠和大鼠的多发性肌炎 - 硬皮病100 kDa自身抗原基因有一个额外的外显子。我们发现有证据支持这样的假说，即MASP2基因内的MAp19特异性外显子可能起源于一个转座元件。在MASP2基因和TARDBP基因中发现了高度保守的内含子序列块。对小鼠和大鼠中MASP2基因座内所有基因的表达进行了分析。MASP - 2和MAp19 mRNA在肝脏中的限制性表达与所研究的所有相邻基因的普遍表达形成对比。
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