Vinogradov Alexander E
Institute of Cytology, Russian Academy of Sciences St Petersburg 194064, Russia.
Nucleic Acids Res. 2005 Jan 26;33(2):559-63. doi: 10.1093/nar/gki184. Print 2005.
The nucleosome formation potential of introns, intergenic spacers and exons of human genes is shown here to negatively correlate with among-tissues breadth of gene expression. The nucleosome formation potential is also found to negatively correlate with the GC content of genomic sequences; the slope of regression line is steeper in exons compared with noncoding DNA (introns and intergenic spacers). The correlation with GC content is independent of sequence length; in turn, the nucleosome formation potential of introns and intergenic spacers positively (albeit weakly) correlates with sequence length independently of GC content. These findings help explain the functional significance of the isochores (regions differing in GC content) in the human genome as a result of optimization of genomic structure for epigenetic complexity and support the notion that noncoding DNA is important for orderly chromatin condensation and chromatin-mediated suppression of tissue-specific genes.
本文显示，人类基因的内含子、基因间间隔区和外显子的核小体形成潜力与基因表达的组织间广度呈负相关。还发现核小体形成潜力与基因组序列的GC含量呈负相关；与非编码DNA（内含子和基因间间隔区）相比，外显子中回归线的斜率更陡。与GC含量的相关性与序列长度无关；反过来，内含子和基因间间隔区的核小体形成潜力与序列长度呈正相关（尽管较弱），且与GC含量无关。这些发现有助于解释人类基因组中等位基因带（GC含量不同的区域）的功能意义，这是基因组结构为表观遗传复杂性而优化的结果，并支持非编码DNA对有序染色质凝聚和染色质介导的组织特异性基因抑制很重要这一观点。
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