Schmitt David A, Cheng Hsiaopo, Maleki Soheila J, Burks A Wesley
US Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA 70124, USA.
J AOAC Int. 2004 Nov-Dec;87(6):1492-7.
Allergies to peanuts are becoming an increasingly important health problem as a result of the persistence and severity of the reaction in allergic individuals. Because no treatment currently is available, avoidance is the only option for peanut-allergic individuals. Avoidance of an abundant and often disguised food such as peanuts, however, is very difficult; therefore, competitive inhibition ELISAs were developed to detect and quantitate each of the major peanut allergens, Ara h 1 and Ara h 2. Under optimal conditions for each assay, the sensitivity of the Ara h 1 and Ara h 2 detection assays were 12 and 0.5 ng/mL, respectively. These assays were primarily devised to effectively compare the levels of Ara h 1 and Ara h 2 in a wide variety of peanuts or peanut products but can also be used to identify cross-reactive antigens. The method is simple and rapid, requiring only one allergen-specific antibody and, therefore, could be adapted specifically to detect the presence of these individual allergens in different foods.
由于过敏个体反应的持续性和严重性，花生过敏正成为一个日益重要的健康问题。由于目前尚无治疗方法，避免接触花生是花生过敏个体的唯一选择。然而，要避免食用像花生这样丰富且常常难以辨认的食物非常困难；因此，人们开发了竞争性抑制酶联免疫吸附测定法（ELISA）来检测和定量每种主要的花生过敏原，即花生球蛋白1（Ara h 1）和花生球蛋白2（Ara h 2）。在每种检测的最佳条件下，Ara h 1和Ara h 2检测测定法的灵敏度分别为12和0.5纳克/毫升。这些测定法主要用于有效比较各种花生或花生制品中Ara h 1和Ara h 2的含量，但也可用于鉴定交叉反应性抗原。该方法简单快速，只需要一种过敏原特异性抗体，因此可以专门用于检测不同食物中这些个别过敏原的存在。
