Gutiérrez M, Soriano V, Oliván M, Lago M, Puente S, Baquero M, González-Lahoz J M
Servicio de Microbiología, Instituto de Salud Carlos III, Madrid.
An Med Interna. 1992 Mar;9(3):111-5.
The presence of antibodies against HIV-2 was analyzed in serum samples from high-risk individuals for retrovirus infection, who were seropositives for HIV-1. Out of a total of 842 samples, 344 presented positive absorbency and 16, threshold ranges, in an enzymatic immunoassay (EIA) designed to detect simultaneously antibodies against HIV-1 and HIV-2. The Western Blot (WB) analysis confirmed the presence of antibodies against HIV-1 in 324 samples with positive or threshold EIA, while it was indetermined in 15 samples. WB was negative in 11 samples. In the 15 samples with indetermined WB and in the 16 samples with threshold EIA, the presence of antibodies against HIV-2 was investigated with an specific WB and a test designed with synthetic peptides (Pepti-lav). Three samples met the isolated seropositivity criteria for HIV-2. Those samples were from 3 african immigrants living in Madrid, which, until now, were asymptomatic, although one of them presented biological signs of immunodeficiency. None of them referred high-risk infectious practices, such a homosexuality or drug-addiction, although all of them referred heterosexual contacts in their native countries. Samples from native subjects meeting serum positivity criteria for HIV-2 were not identified.
对感染逆转录病毒的高危个体（HIV-1血清阳性）的血清样本进行了抗HIV-2抗体检测。在总共842份样本中，在一项旨在同时检测抗HIV-1和抗HIV-2抗体的酶免疫测定（EIA）中，有344份呈现阳性吸光度，16份处于临界范围。蛋白质印迹法（WB）分析证实，324份EIA呈阳性或临界值的样本中存在抗HIV-1抗体，15份样本结果不确定。11份样本的WB检测为阴性。在15份WB结果不确定的样本和16份EIA处于临界值的样本中，采用特异性WB和合成肽设计的检测方法（Pepti-lav）检测抗HIV-2抗体的存在情况。有3份样本符合HIV-2单独血清阳性标准。这些样本来自居住在马德里的3名非洲移民，到目前为止他们没有症状，尽管其中一人有免疫缺陷的生物学迹象。他们均未提及有高危感染行为，如同性恋或吸毒，尽管他们都提到在本国存在异性接触。未发现符合HIV-2血清阳性标准的本国受试者样本。
