Błazewicz Jacek, Formanowicz Piotr
Institute of Computing Science, Poznań University of Technology, Piotrowo 3A, 60-965 Poznań, Poland.
Comput Biol Chem. 2005 Feb;29(1):69-77. doi: 10.1016/j.compbiolchem.2004.12.001.
Despite the impressive progress in many areas of biological sciences reading DNA sequences still remains one of the most important problems. One of the methods of DNA sequencing is sequencing by hybridization which is composed of two stages--the biochemical one and the computational one. Although this method is quite modern it suffers sensitivity to errors appearing in the biochemical stage. This is a motivation for developing some new variants of the method which should be more resistant to errors of these types. Two of such non-standard approaches are multistage and isothermic sequencing by hybridization. Each of the methods is less sensitive to some types of the errors appearing in the biochemical stage, in comparison to the standard version of the method, but on the other hand, they are more sensitive to remaining types of errors. However, a combination of the two approaches reduces the sensitivity of the components.
尽管在生物科学的许多领域都取得了令人瞩目的进展，但读取DNA序列仍然是最重要的问题之一。DNA测序的方法之一是杂交测序，它由两个阶段组成——生化阶段和计算阶段。虽然这种方法相当现代，但它对生化阶段出现的错误很敏感。这促使人们开发该方法的一些新变体，使其对这类错误更具抗性。两种这样的非标准方法是多阶段杂交测序和等温杂交测序。与该方法的标准版本相比，每种方法对生化阶段出现的某些类型的错误不太敏感，但另一方面，它们对其余类型的错误更敏感。然而，将这两种方法结合起来可以降低各组件的敏感性。
