Hennessy T P, Quaglia M, Kornysova O, Grimes B A, Lubda D, Unger K K
Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, Duesbergweg 10-14, D-55099 Mainz, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Mar 5;817(1):127-37. doi: 10.1016/j.jchromb.2004.10.043.
Complex biological samples require very high resolution separation strategies. The platform introduced here capitalises on the hyphenation of liquid chromatographic (LC) and electric potential gradient electrochromatographic multi-dimensional separation genres. First-dimension selectivity is provided by simultaneous size exclusion (SEC) and strong cation exchange (SCX) chromatography modes, while the second dimension comprises reversed phase (RP) characteristics in a dynamic (time-variant) electric field. The time-variant potential gradient with reversal of polarity is applied across the second dimension monolithic capillary throughout the duration of the solvent strength gradient elution. Hence, the platform offers comprehensive on-line sample clean-up (matrix depletion, analyte enrichment), fractionation (first dimention LC), and separation (second dimension LC) with the prospect of altering selectivity via polarity reversal dynamic electric field tuning.
复杂的生物样品需要非常高分辨率的分离策略。这里介绍的平台利用了液相色谱（LC）和电势梯度电色谱多维分离类型的联用。第一维选择性由同时进行的尺寸排阻（SEC）和强阳离子交换（SCX）色谱模式提供，而第二维则在动态（随时间变化）电场中具有反相（RP）特性。在溶剂强度梯度洗脱的整个过程中，在第二维整体毛细管上施加具有极性反转的随时间变化的电势梯度。因此，该平台提供了全面的在线样品净化（基质去除、分析物富集）、分级分离（第一维LC）和分离（第二维LC），并有望通过极性反转动态电场调节来改变选择性。
