Bowman Gregory D, Goedken Eric R, Kazmirski Steven L, O'Donnell Mike, Kuriyan John
Departments of Molecular and Cell Biology and Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
FEBS Lett. 2005 Feb 7;579(4):863-7. doi: 10.1016/j.febslet.2004.11.038.
Clamp loaders are heteropentameric ATPase assemblies that load sliding clamps onto DNA and are critical for processive DNA replication. The DNA targets for clamp loading are double-stranded/single-stranded junctions with recessed 3' ends (primer-template junctions). Here, we briefly review the crystal structures of clamp loader complexes and the insights they have provided into the mechanism of the clamp loading process.
夹子加载器是异源五聚体ATP酶组装体，可将滑动夹子加载到DNA上，对连续性DNA复制至关重要。夹子加载的DNA靶点是具有凹陷3'端的双链/单链连接（引物-模板连接）。在这里，我们简要回顾夹子加载器复合物的晶体结构以及它们为夹子加载过程机制所提供的见解。
