Stillman Bruce
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
FEBS Lett. 2005 Feb 7;579(4):877-84. doi: 10.1016/j.febslet.2004.12.011.
Prior to the initiation of DNA replication, chromosomes must establish a biochemical mark that permits the recruitment in S phase of the DNA replication machinery that copies DNA. The process of chromosome replication in eukaryotes also must be coordinated with segregation of the duplicated chromosomes to daughter cells during mitosis. Protein complexes that utilize ATP coordinate events at origins of DNA replication and later they participate in the initiation of DNA replication. In eukaryotes, some of these proteins also play a part in later processes that ensure accurate inheritance of chromosomes in mitosis, including spindle attachment of chromosomes, accurate duplication of centrosomes and cytokinesis. A perspective of how ATP-dependent proteins accomplish this task in eukaryotes is discussed.
在DNA复制开始之前，染色体必须建立一种生化标记，以便在S期招募复制DNA的DNA复制机制。真核生物中染色体复制的过程还必须与有丝分裂期间将复制后的染色体分离到子细胞的过程相协调。利用ATP的蛋白质复合物协调DNA复制起点处的事件，随后它们参与DNA复制的起始。在真核生物中，其中一些蛋白质还在确保有丝分裂中染色体精确遗传的后续过程中发挥作用，包括染色体的纺锤体附着、中心体的精确复制和胞质分裂。本文讨论了ATP依赖蛋白在真核生物中如何完成这项任务的观点。
