Roeder Robert G
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
FEBS Lett. 2005 Feb 7;579(4):909-15. doi: 10.1016/j.febslet.2004.12.007.
Transcriptional regulation in eukaryotes involves structurally and functionally distinct nuclear RNA polymerases, corresponding general initiation factors, gene-specific (DNA-binding) regulatory factors, and a variety of coregulatory factors that act either through chromatin modifications (e.g. histone acetyltransferases and methyltransferases) or more directly (e.g. Mediator) to facilitate formation and function of the preinitiation complex. Biochemical studies with purified factors and DNA versus recombinant chromatin templates have provided insights into the nature and mechanism of action of these factors, including pathways for their sequential function in chromatin remodeling and preinitiation complex formation/function (transcription) steps and a possible role in facilitating the transition between these steps.
真核生物中的转录调控涉及结构和功能上不同的核RNA聚合酶、相应的通用起始因子、基因特异性（DNA结合）调控因子以及多种共调控因子，这些共调控因子通过染色质修饰（如组蛋白乙酰转移酶和甲基转移酶）或更直接地（如中介体）发挥作用，以促进起始前复合物的形成和功能。使用纯化因子和DNA与重组染色质模板进行的生化研究，为这些因子的性质和作用机制提供了见解，包括它们在染色质重塑和起始前复合物形成/功能（转录）步骤中的顺序功能途径，以及在促进这些步骤之间转换中可能发挥的作用。
