Kurauchi Tomomi, Yokota Kenji, Matsuo Toshihiko, Fujinami Yoshihito, Isogai Emiko, Isogai Hiroshi, Ohtsuki Hiroshi, Oguma Keiji
Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikatacho, Okayama 700-8558, Japan.
FEMS Immunol Med Microbiol. 2005 Feb 1;43(2):125-31. doi: 10.1016/j.femsim.2004.07.007.
Immune reactions against microorganisms play an important pathogenic role in Adamantiades-Behçet's disease. We had previously obtained Streptococcus sanguinis (strain BD113-20), isolated from the oral cavity of patients with Adamantiades-Behçet's syndrome. To investigate the pathogenesis of this isolate, we examined neutrophil reactions and levels of cytokine production by lymphocytes after stimulation with the strain. The reactions of neutrophils were examined by chemiluminescence assay using whole blood. The amounts of interferon gamma (IFN-gamma) and interleukin (IL)-4, IL-8, IL-10 and IL-12, produced by peripheral blood mononuclear cells, were measured by ELISA. Strain BD113-20 activated neutrophils from Adamantiades-Behçet's patients and healthy volunteers, and, in addition it increased the IFN-gamma production by lymphocytes. Lymphocytes from Adamantiades-Behçet's patients showed a dominant T helper-1 immune response. Results indicated that both bacterial stimulation and host hypersensitivity might be involved in the symptoms and pathogenesis of Adamantiades-Behçet's disease.
针对微生物的免疫反应在白塞病中起着重要的致病作用。我们之前从白塞综合征患者口腔中分离出了血链球菌（菌株BD113 - 20）。为了研究该分离菌株的发病机制，我们检测了用该菌株刺激后中性粒细胞的反应以及淋巴细胞产生细胞因子的水平。使用全血通过化学发光分析法检测中性粒细胞的反应。通过酶联免疫吸附测定法测量外周血单核细胞产生的干扰素γ（IFN - γ）、白细胞介素（IL）-4、IL - 8、IL - 10和IL - 12的量。菌株BD113 - 20激活了白塞病患者和健康志愿者的中性粒细胞，此外，它还增加了淋巴细胞产生的IFN - γ。白塞病患者的淋巴细胞表现出占主导地位的辅助性T细胞1型免疫反应。结果表明，细菌刺激和宿主超敏反应可能都与白塞病的症状和发病机制有关。
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