Rick John, Chou Tse-Chuan
Chemical Engineering Department, National Cheng Kung University, Tainan 70101, Taiwan.
Biosens Bioelectron. 2005 Mar 15;20(9):1878-83. doi: 10.1016/j.bios.2004.11.015.
Molecularly imprinted thin films consisting of proteins embedded in polymerised aminophenyl boronic acid have been made on glass supports. The protein contents of the films have been optimised to achieve a maximum energy of interaction between the film and the native template. The fabrication of the films and the subsequent removal from their surfaces of the imprint proteins has been shown to be a facile and easily reproduced process. The enthalpy changes associated with the rebinding of the films with their original templates (lysozyme and cytochrome c) and with non-native templates has been examined by micro-calorimetry. The results demonstrate that thin films can be successfully imprinted as shown by the significant reduction in the enthalpy (DeltaH) observed when the films were rebound with proteins other than the original templates. Additionally, it was shown that after binding, non-template proteins could be removed by washing and a greater enthalpy again observed when the films were rebound with the native protein compared to that which had been found with the non-native protein.
由嵌入聚合氨基苯硼酸中的蛋白质组成的分子印迹薄膜已制备在玻璃载体上。薄膜的蛋白质含量已得到优化,以实现薄膜与天然模板之间的最大相互作用能。薄膜的制备以及随后从其表面去除印迹蛋白质已被证明是一个简便且易于重复的过程。通过微量热法研究了薄膜与其原始模板(溶菌酶和细胞色素c)以及非天然模板重新结合时的焓变。结果表明,当薄膜与原始模板以外的蛋白质重新结合时,焓(ΔH)显著降低,这表明薄膜可以成功印迹。此外,研究表明,结合后,通过洗涤可以去除非模板蛋白质,并且当薄膜与天然蛋白质重新结合时,与非天然蛋白质相比,再次观察到更大的焓。