Turner Nicholas W, Jeans Christopher W, Brain Keith R, Allender Christopher J, Hlady Vladimir, Britt David W
Cranfield Health, Cranfield University at Silsoe, Silsoe MK45 4DT, UK.
Biotechnol Prog. 2006 Nov-Dec;22(6):1474-89. doi: 10.1021/bp060122g.
Molecular imprinting is a generic technology that allows for the introduction of sites of specific molecular affinity into otherwise homogeneous polymeric matrices. Commonly this technique has been shown to be effective when targeting small molecules of molecular weight <1500, while extending the technique to larger molecules such as proteins has proven difficult. A number of key inherent problems in protein imprinting have been identified, including permanent entrapment, poor mass transfer, denaturation, and heterogeneity in binding pocket affinity, which have been addressed using a variety of approaches. This review focuses on protein imprinting in its various forms, ranging from conventional bulk techniques to novel thin film and monolayer surface imprinting approaches.
分子印迹是一种通用技术，可将特定分子亲和力位点引入原本均相的聚合物基质中。通常，当靶向分子量小于1500的小分子时，该技术已被证明是有效的，而将该技术扩展到更大的分子（如蛋白质）则被证明很困难。蛋白质印迹中已发现一些关键的固有问题，包括永久性截留、传质不良、变性以及结合口袋亲和力的异质性，已使用多种方法来解决这些问题。本综述重点关注蛋白质印迹的各种形式，从传统的本体技术到新型的薄膜和单层表面印迹方法。
