Xu Li Xiao, Hui Ni, Ma Li Ying, Wang Huai You
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2005 Mar;61(5):855-9. doi: 10.1016/j.saa.2004.06.016.
The property of carvedilol in acid-alkaline medium was studied by spectrofluorimetry. Effects of organic solvents on carvedilol fluorescence spectra were examined and the reason was discussed. A simple, rapid and high sensitive fluorimetric method for the determination of carvedilol in medicine is developed. The measurement of relative fluorescence intensity was carried out at 356 nm with excitation at 254 nm. Effects of pH, standing time and foreign ions on the determination of carvedilol have been examined. A linear relationship was obtained between the relative fluorescence intensity and concentration of carvedilol in the range of 0.50-270 ng ml(-1). The linear regression equation of the calibration graph is C = 0.000543F - 0.002 (C is concentration (microg ml(-1)) and F is relative fluorescence intensity in the equation), with a correlation coefficient of linear regression of 0.9998 and relative standard deviation of 2.31%. The detection limit of this method is 0.19 ng ml(-1), and recovery is from 98.70 to 102.1%. This method can be used for determination of carvedilol in tablet. The results obtained by this method agreed with those by the official method.
采用荧光分光光度法研究了卡维地洛在酸碱介质中的性质。考察了有机溶剂对卡维地洛荧光光谱的影响并探讨了原因。建立了一种简单、快速、高灵敏度的荧光分析法用于测定药物中的卡维地洛。在激发波长为254nm时，于356nm处测量相对荧光强度。考察了pH值、静置时间和外来离子对卡维地洛测定的影响。卡维地洛浓度在0.50 - 270 ng ml⁻¹范围内，相对荧光强度与浓度呈线性关系。校准曲线的线性回归方程为C = 0.000543F - 0.002（式中C为浓度（μg ml⁻¹），F为相对荧光强度），线性回归相关系数为0.9998，相对标准偏差为2.31%。该方法的检测限为0.19 ng ml⁻¹，回收率为98.70% - 102.1%。该方法可用于片剂中卡维地洛的测定。该方法所得结果与法定方法一致。
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