甘薯烟粉虱（Bemisia tabaci）和温室白粉虱（Trialeurodes vaporariorum）蜕皮激素的鉴定。

Identification of the molting hormone of the sweet potato (Bemisia tabaci) and greenhouse (Trialeurodes vaporariorum) whitefly.

作者信息

Gelman Dale B, Blackburn Michael B, Hu Jing S

机构信息

Insect Biocontrol Laboratory, USDA, ARS, PSI, Building 011A, Room 214, BARC West, Beltsville, MD 20705, USA.

出版信息

J Insect Physiol. 2005 Jan;51(1):47-53. doi: 10.1016/j.jinsphys.2004.11.003.

DOI:10.1016/j.jinsphys.2004.11.003

PMID:15686645

Abstract

In order to identify the whitefly molting hormone, whole body extracts of mature 4th instar and newly formed pharate adult Bemisia tabaci (Biotype B) and Trialeurodes vaporariorum were prepared and subjected to reverse phase high performance liquid chromatography (RPHPLC). Ecdysteroid content of fractions was determined by enzymeimmunoassay (EIA). The only detectable ecdysteroids that were present in significant amounts in whitefly extracts were ecdysone and 20-hydroxyecdysone. The concentrations of 20-hydroxyecdysone in B. tabaci and T. vaporariorum extracts, respectively, were 40 and 15 times greater than the concentrations of ecdysone. The identity of the two ecdysteroids was confirmed by normal phase high performance liquid chromatography (NPHPLC). When ecdysteroid content of RPHPLC fractions was assayed by radioimmunoassay (RIA), small amounts of polar ecdysteroids were also detected indicating that these ecdysteroids have a very low affinity for the antiserum used in the EIA. Ecdysteroid at 10.4 mM administered by feeding stimulated 2nd instar whitefly nymphs to molt. Based on our results, it appears that 20-hydroxyecdysone is the whitefly molting hormone.

摘要

为了鉴定粉虱蜕皮激素，制备了烟粉虱（生物型B）和温室白粉虱成熟四龄若虫及新形成的伪成虫的全虫提取物，并进行反相高效液相色谱（RPHPLC）分析。通过酶免疫测定法（EIA）测定各馏分中的蜕皮甾类含量。粉虱提取物中大量存在的唯一可检测到的蜕皮甾类是蜕皮激素和20-羟基蜕皮激素。烟粉虱和温室白粉虱提取物中20-羟基蜕皮激素的浓度分别比蜕皮激素的浓度高40倍和15倍。通过正相高效液相色谱（NPHPLC）确认了这两种蜕皮甾类的身份。当用放射免疫测定法（RIA）检测RPHPLC馏分中的蜕皮甾类含量时，还检测到少量极性蜕皮甾类，表明这些蜕皮甾类与EIA中使用的抗血清亲和力很低。通过喂食给予10.4 mM的蜕皮甾类可刺激二龄粉虱若虫蜕皮。根据我们的结果，似乎20-羟基蜕皮激素是粉虱蜕皮激素。