Tomaschko K H, Bückmann D
Abteilung Allgemeine Zoologie, Universität Ulm, Germany.
Gen Comp Endocrinol. 1993 Jun;90(3):296-305. doi: 10.1006/gcen.1993.1085.
In embryos, larvae, and juveniles of Pycnogonum litorale, unusual ecdysteroids have been found at excessively high levels. Concentrations and dynamics of the following eight ecdysteroids were determined by HPLC: 20-hydroxyecdysone, 20-hydroxyecdysone 22-glycolate, (25R) and (25S) isomers of 20,26-dihydroxyecdysone 22-acetate, 22-deoxy-20,26-dihydroxyecdysone, 20-hydroxyecdysone 22-acetate, 22-deoxy-20-hydroxyecdysone, and ecdysone 22-glycolate. The excessively high content of ecdysteroids is found in all developmental stages. Juvenile pycnogonids contain the highest total ecdysteroid amounts ever found in arthropods: 0.1% of their body dry weight. 20-Hydroxyecdysone 22-acetate is the prominent ecdysteroid and represents at all stages of both sexes, 66.2-85.8% of the total ecdysteroids. This compound, as well as all other ecdysteroids without a free 22-OH group, are presumed to be inactive as molting hormones. In contrast to these compounds, 20-hydroxyecdysone is present only in amounts similar to those in other arthropods. Furthermore, it is the only ecdysteroid with its peak at apolysis. It is regarded as the molt-promoting hormone. The origin and function of the other ecdysteroids in different developmental stages are discussed.
在海滨雄蟹的胚胎、幼虫和幼体中，发现了异常高水平的蜕皮甾体。通过高效液相色谱法测定了以下八种蜕皮甾体的浓度和动态变化：20-羟基蜕皮酮、20-羟基蜕皮酮22-乙醇酸酯、20,26-二羟基蜕皮酮22-乙酸酯的(25R)和(25S)异构体、22-脱氧-20,26-二羟基蜕皮酮、20-羟基蜕皮酮22-乙酸酯、22-脱氧-20-羟基蜕皮酮和蜕皮酮22-乙醇酸酯。在所有发育阶段都发现了蜕皮甾体的过高含量。幼年雄蟹含有节肢动物中迄今发现的最高总蜕皮甾体量：占其身体干重的0.1%。20-羟基蜕皮酮22-乙酸酯是主要的蜕皮甾体，在两性的所有阶段均占总蜕皮甾体的66.2-85.8%。该化合物以及所有其他没有游离22-OH基团的蜕皮甾体，被认为作为蜕皮激素是无活性的。与这些化合物相反，20-羟基蜕皮酮的含量仅与其他节肢动物中的含量相似。此外，它是唯一在蜕皮前期达到峰值的蜕皮甾体。它被视为促进蜕皮的激素。讨论了不同发育阶段其他蜕皮甾体的来源和功能。
