携带天然N-聚糖的CD52糖蛋白的化学酶法合成。

Chemoenzymatic synthesis of CD52 glycoproteins carrying native N-glycans.

作者信息

Li Hengguang, Singh Suddham, Zeng Ying, Song Haijing, Wang Lai-Xi

机构信息

Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, 725 West Lombard Street, Baltimore, MD 21201, USA.

出版信息

Bioorg Med Chem Lett. 2005 Feb 15;15(4):895-8. doi: 10.1016/j.bmcl.2004.12.066.

DOI:10.1016/j.bmcl.2004.12.066

PMID:15686882

Abstract

A facile synthesis of homogeneous CD52 glycoproteins carrying native N-glycans was achieved using an endolycosidase-catalyzed oligosaccharide transfer as the key step. The synthesis consists of two steps: the solid phase synthesis of GlcNAc-CD52 and the transfer of a high-mannose type or complex type N-glycan from Man(9)GlcNAc(2) Asn or a sialglycopeptide to the GlcNAc-CD52, under the catalysis of the endo-beta-N-acetylglucosaminidases from Arthrobacter (Endo-A) and Mucor hiemalis (Endo-M), respectively.

摘要

通过以内切糖苷酶催化的寡糖转移为关键步骤，实现了携带天然N-聚糖的均一性CD52糖蛋白的简便合成。该合成包括两个步骤：GlcNAc-CD52的固相合成，以及分别在节杆菌（Endo-A）和冬黑麂霉（Endo-M）的β-N-乙酰氨基葡萄糖苷酶催化下，将高甘露糖型或复合型N-聚糖从Man(9)GlcNAc(2) Asn或唾液酸糖肽转移至GlcNAc-CD52。