Kim Seong-Gon, Yang Byoung-Eun, Oh So-Hee, Min Soo-Kee, Hong Sam-Pyo, Choi Je-Yong
Department of Oral and Maxillofacial Surgery, College of Medicine, Hallym University, Anyang, Korea.
J Oral Pathol Med. 2005 Mar;34(3):178-83. doi: 10.1111/j.1600-0714.2004.00285.x.
Bone morphogenic protein-4 (BMP-4) is widely expressed in oral cavity and involved in tooth morphogenesis, cellular differentiation and proliferation. The purpose of this study was to compare the difference in expression pattern of BMP-4 in odontogenic keratocysts (OKC) and dentigerous cysts (DC).
We evaluated 77 cysts, OKC (n = 34) or DC (n = 43). The average age of patients with OKC was 29.5 +/- 14.4 and that of patients with DC was 36.1 +/- 19.4. The male to female ratio was 20:14 for OKC and 27:16 for DC. Ten cases of OKC were recurrences. Expression of BMP-4 was determined by immunohistochemistry and in situ hybridization.
The intensity scales were (-) for invisible or trace staining, (+) for visible staining, and (++) for dense, strong staining. OKCs exhibited the following staining patterns: the epithelium in 15/34 specimens and the mesenchymal cells in 17/34 specimens showed (++) stain. In contrast, the staining pattern of DC was (-) for epithelium in 37/43 specimens. The mesenchymal cells showed (-) degree staining in 30/43 specimens. The difference between the groups studied was significant (P < 0.001 in epithelium and mesenchymal cells). When recurrent and non-recurrent OKC were compared BMP-4 was expressed more intensely in the recurrent cases (P = 0.036 in epithelium). The difference in BMP-4 expression in mesenchymal cells was not significant. In situ hybridization demonstrated positive mRNA probes to BMP-4 were localized in epithelium and mesenchymal cells of OKCs and DCs.
BMP-4 was expressed more intensely in OKC when compared with DC, and was more intensely expressed in recurrent cases.
骨形态发生蛋白-4（BMP-4）在口腔中广泛表达，参与牙齿形态发生、细胞分化和增殖。本研究的目的是比较BMP-4在牙源性角化囊肿（OKC）和含牙囊肿（DC）中的表达模式差异。
我们评估了77个囊肿，其中OKC（n = 34）或DC（n = 43）。OKC患者的平均年龄为29.5±14.4岁，DC患者的平均年龄为36.1±19.4岁。OKC的男女比例为20:14，DC的男女比例为27:16。10例OKC为复发病例。通过免疫组织化学和原位杂交检测BMP-4的表达。
强度分级为：不可见或微量染色为（-），可见染色为（+），浓密、强染色为（++）。OKC呈现以下染色模式：在34个标本中的15个标本的上皮和34个标本中的17个标本的间充质细胞显示（++）染色。相比之下，DC的染色模式为：在43个标本中的37个标本的上皮为（-）染色。在43个标本中的30个标本的间充质细胞显示（-）度染色。所研究组之间的差异具有显著性（上皮和间充质细胞中P < 0.001）。当比较复发性和非复发性OKC时，BMP-4在复发病例中的表达更强烈（上皮中P = 0.036）。间充质细胞中BMP-4表达的差异不显著。原位杂交显示，BMP-4的阳性mRNA探针定位于OKC和DC的上皮和间充质细胞中。
与DC相比，BMP-4在OKC中的表达更强烈，且在复发病例中表达更强烈。
