Soogarun Suphan, Suwansaksri Jamsai, Wiwanitkit Viroj
Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2004 Jun;35(2):267-9.
Third-stage larvae were used as antigen in the diagnosis of gnathostomiasis in Western blot analysis. Normally, the larvae were obtained from digestion of eel's liver (Fluta alba) by the enzyme pepsin. We used pineapple juice (Ananus comosus) instead of enzyme pepsin in harvesting Gnathostoma spinigerum third-stage larvae. The difference in recovered larvae numbers, between pineapple juice and pepsin, were not statistically significantly different (p>0.05). The larvae from pepsin and pineapple juice digestion were cultivated on BME for 7 days; the survival rates were not significantly different (p>0.05). Thus, pineapple juice is another enzyme of choice for recovering Gnathostoma spinigerum third-stage larvae.
三期幼虫被用作抗原，用于免疫印迹分析诊断颚口线虫病。通常，幼虫是通过用胃蛋白酶消化鳗鱼肝脏（白鳝）获得的。我们在收集棘颚口线虫三期幼虫时使用菠萝汁（凤梨）代替胃蛋白酶。菠萝汁和胃蛋白酶回收的幼虫数量差异无统计学意义（p>0.05）。用胃蛋白酶和菠萝汁消化得到的幼虫在基础培养基 Eagle 上培养 7 天；存活率差异无统计学意义（p>0.05）。因此，菠萝汁是另一种用于回收棘颚口线虫三期幼虫的首选酶。
