[应用生物胶粘贴技术原位移植构建人胃癌转移裸鼠模型]

[Nude mouse model of human gastric carcinoma metastasis constructed by orthotopic transplantation using organism glue paste technique].

作者信息

Chen Ya-Lin, Wei Pin-Kang, Xu Ling, Su Xiao-Mei

机构信息

Department of Traditional Chinese Medicine, Changzheng Hospital, The Second Military Medical University, Shanghai, 200003, P. R. China.

出版信息

Ai Zheng. 2005 Feb;24(2):246-8.

PMID:15694045

Abstract

BACKGROUND & OBJECTIVE: Ideal animal model of tumor metastasis is an important precondition for the research on tumor prevention and treatment. Traditional gastric cancer models were constructed using full tumor tissue block by "subcutaneous transplantation" or "gastric bursa method" with limitations, such as operative complexity, and low survival rate. We tried to construct nude mouse model of human gastric carcinoma metastasis by orthotopic transplantation using organism glue paste technique to solve the problems.

METHODS

Transplanted gastric tumor tissue block was constructed through repeatedly subcutaneously inoculating and passaging human gastric carcinoma SGC-7901 cells in nude mouse, and pasted orthotopically on the stomach wall of BALB/c nude mouse with OB biological glue. Tumor growth, successful rate of orthotopic transplantation, and incidence of metastasis in nude mice performed organism glue paste were compared with those in nude mice performed subcutaneous transplantation or gastric bursa method.

RESULTS

In nude mice performed organism glue paste, the successful rate of orthotopic transplantation was 100%, the metastasis rate of local lymph node, lung, liver, and peritoneum were 100%, 62.5%, 87.5%, and 87.5%, respectively, while those in nude mice performed gastric bursa method were 100%, 100%, 50.0%, 50.0%, and 33.3%, respectively. The nude mice performed organism glue paste showed a increased metastatic rate of peritoneum (P < 0.05) with a higher survival rate. No local invasion and distance metastasis was observed in nude mice performed subcutaneous transplantation.

CONCLUSION

Organism glue paste technique can create gastric cancer metastasis model more easily than traditional methods, and represent clinical metastasis process.

摘要

背景与目的

理想的肿瘤转移动物模型是肿瘤防治研究的重要前提。传统胃癌模型采用“皮下移植”或“胃囊法”，利用完整肿瘤组织块构建，存在手术复杂、生存率低等局限性。我们尝试采用生物胶粘贴技术原位移植构建人胃癌转移裸鼠模型以解决这些问题。

方法

通过在裸鼠体内反复皮下接种和传代人胃癌SGC-7901细胞构建移植性胃肿瘤组织块，并用OB生物胶将其原位粘贴于BALB/c裸鼠胃壁。将采用生物胶粘贴法的裸鼠与采用皮下移植法或胃囊法的裸鼠在肿瘤生长情况、原位移植成功率及转移发生率等方面进行比较。

结果

采用生物胶粘贴法的裸鼠原位移植成功率为100%，局部淋巴结、肺、肝及腹膜的转移率分别为100%、62.5%、87.5%和87.5%；而采用胃囊法的裸鼠原位移植成功率为100%，局部淋巴结、肺、肝及腹膜的转移率分别为100%、50.0%、50.0%和33.3%。采用生物胶粘贴法的裸鼠腹膜转移率升高（P<0.05），生存率更高。采用皮下移植法的裸鼠未观察到局部侵袭和远处转移。