ExPrimer：从外显子-外显子连接区设计引物。
ExPrimer: to design primers from exon--exon junctions.
作者信息
Sandhu Kuljeet S, Acharya Kshitish K
机构信息
Institute of Bioinformatics and Applied Biotechnology (IBAB) G-05, Tech Park Mall, ITPL, Bangalore, India.
出版信息
Bioinformatics. 2005 May 1;21(9):2091-2. doi: 10.1093/bioinformatics/bti304. Epub 2005 Feb 4.
SUMMARY
ExPrimer is a web-based computer program to design primers mainly from a specified exon-exon junction (E-E-jn) of a gene of interest. The tool suggests the optimum primer-pair(s) of which the right (reverse) primer represents a particular E-E-jn of the mRNA. The 'product length' decides the location of the left primer. The results also include all other primer pairs considered and their 'scores'. ExPrimer can use the NCBI BLASTn program for sequence specificity of primers. The tool is useful in many areas of molecular biology research that involve hybridization of short sequences with mRNA or cDNA.
AVAILABILITY
摘要
ExPrimer是一个基于网络的计算机程序，主要用于从感兴趣基因的特定外显子-外显子连接区（E-E-jn）设计引物。该工具会推荐最佳引物对，其中右侧（反向）引物代表mRNA的特定E-E-jn。“产物长度”决定左侧引物的位置。结果还包括所有考虑的其他引物对及其“分数”。ExPrimer可以使用NCBI BLASTn程序来检测引物的序列特异性。该工具在分子生物学研究的许多领域都很有用，这些领域涉及短序列与mRNA或cDNA的杂交。
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