Veronese F M, Schiavon O, Bevilacqua R, Rodighiero G
Z Naturforsch C Biosci. 1979 May-Jun;34C(5-6):392-6. doi: 10.1515/znc-1979-5-612.
For a better insight of the molecular basis of the photobiological effects of furocoumarins, the relevance of proteins oxydation by singlet oxygen produced by these substrates under irradiation with long u. v. light was studied. Complex oligomeric as well as simple monomeric purified enzymes with high or low molecular weight and different properties and simple amino acids were irradiated under oxygen in presence of 8-methoxy-psoralen. The effects on both proteins and amino acids were compared with those obtained under similar conditions with typical photosensitizers as methylene blue and Rose Bengal. The results indicated that the photooxydation of proteins, although possible, appears to play a minor role, if any, in the mechanism of action of furocoumarin.
为了更深入了解补骨脂素光生物学效应的分子基础，研究了这些底物在长波紫外线照射下产生的单线态氧对蛋白质氧化的相关性。在8-甲氧基补骨脂素存在的情况下，在氧气中对具有不同性质和简单氨基酸的高分子量或低分子量的复杂寡聚体以及简单单体纯化酶进行辐照。将对蛋白质和氨基酸的影响与在类似条件下使用典型光敏剂（如亚甲蓝和孟加拉玫瑰红）所获得的影响进行比较。结果表明，蛋白质的光氧化虽然是可能的，但在补骨脂素的作用机制中似乎只起次要作用（如果有作用的话）。
