[克拉霉素的极谱催化波及其应用]

[Polarographic catalytic wave of clarithromycin and its applications].

作者信息

Ma Huai-ling, Xu Mao-tian, Song Jun-feng

机构信息

Department of Chemistry, Shangqiu Normal College, Shangqiu 476000, China.

出版信息

Yao Xue Xue Bao. 2004 Oct;39(10):821-5.

PMID:15700824

Abstract

AIM

To develop a new method for the determination of clarithromycin.

METHODS

The catalytic wave of clarithromycin in the presence of K2S2O8 was used for improving the analytical sensitivity. The rapid determination of clarithromycin has been carried out by linear single sweep polarography.

RESULTS

The reduction wave of clarithromycin appeared at ca. -0.79 V (vs SCE) in 0.24 mol x L(-1) KH2PO4-Na2HPO4 (pH 6.81) supporting electrolyte, which was ascribed to the reduction of carbonyl group on C-9 position. In the presence of 0.01 mol x L(-1) K2S2O8, the reduction wave was catalyzed to produce a parallel catalytic wave. The peak current of the catalytic wave was ca. Twenty times higher than that of the corresponding reduction wave. Based on the catalytic wave, a new method for the determination of clarithromycin has been proposed. The peak current of the catalytic wave was rectilinear to clarithromycin concentration in the range of 4.0 x 10(-7)-5.0 x 10(-5) mol x L(-1). The detection limit was 2.0 x 10(-7) mol x L(-1).

CONCLUSION

The proposed method could be used for the direct determination of clarithromycin in pharmaceuticals and urine without preliminary separation.

摘要

目的

开发一种测定克拉霉素的新方法。

方法

利用克拉霉素在K2S2O8存在下的催化波来提高分析灵敏度。采用线性单扫描极谱法对克拉霉素进行快速测定。

结果

在0.24 mol·L-1 KH2PO4-Na2HPO4（pH 6.81）支持电解质中，克拉霉素的还原波出现在约-0.79 V（vs SCE）处，这归因于C-9位羰基的还原。在0.01 mol·L-1 K2S2O8存在下，还原波被催化产生平行催化波。催化波的峰电流约为相应还原波峰电流的20倍。基于此催化波，提出了一种测定克拉霉素的新方法。催化波的峰电流在4.0×10-7 - 5.0×10-5 mol·L-1范围内与克拉霉素浓度呈线性关系。检测限为2.0×10-7 mol·L-1。