Ma Huai-ling, Xu Mao-tian, Qu Peng, Ma Xiao-hua
Department of Chemistry, Shangqiu Normal College, Shangqiu 476000, China.
Yao Xue Xue Bao. 2005 Aug;40(8):750-3.
To establish a polarographic method of parallel catalytic hydrogen wave for determination of glimepiride.
The catalytic wave of glimepiride in the presence of K2S2O8 was used to improve the analytical sensitivity. The rapid determination of glimepiride was done by linear single sweep polarography.
The catalytic hydrogen wave of glimepiride was measured at ca. -1.36 (vs SCE) in 0.09 mol x L(-1) Na2B4O7-KH2PO4 (pH 6.24 +/- 0.1) supporting electrolyte. When 1.0 x 10(-2) mol x L(-1) K2S2O8 was present, the current increased by 25 times, and the peak potentioal was unchanged, producing a more sensitive parallel catalytic hydrogen wave. The peak current of the parallel catalytic hydrogen wave was rectilinear to the glimepiride concentration in the range 1.0 x 10(-7) - 4.2 x 10(-5) mol x L(-1) (r = 0.9990, n = 9). The detection limit was 5.0 x 10(-8) mol x L(-1).
The proposed method could be applied to the determination of glimepiride in pharmaceuticals without preliminary separation.
建立一种平行催化氢波极谱法测定格列美脲。
利用格列美脲在K2S2O8存在下的催化波提高分析灵敏度，采用线性单扫描极谱法快速测定格列美脲。
在0.09 mol·L-1 Na2B4O7-KH2PO4（pH 6.24±0.1）支持电解质中，格列美脲的催化氢波在约-1.36（vs SCE）处测得。当存在1.0×10-2 mol·L-1 K2S2O8时，电流增加25倍，峰电位不变，产生更灵敏的平行催化氢波。平行催化氢波的峰电流在1.0×10-7 - 4.2×10-5 mol·L-1范围内与格列美脲浓度呈线性关系（r = 0.9990，n = 9），检测限为5.0×10-8 mol·L-1。
该方法可不经预分离直接用于药物中格列美脲的测定。
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