通过寡聚N3'→P5'氨基磷酸酯修饰和添加梳型阳离子共聚物实现核酸组装体的协同稳定作用。

Synergistic stabilization of nucleic acid assembly by oligo-N3'-->P5' phosphoramidate modification and additions of comb-type cationic copolymers.

作者信息

Torigoe Hidetaka, Maruyama Atushi

机构信息

Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.

出版信息

J Am Chem Soc. 2005 Feb 16;127(6):1705-10. doi: 10.1021/ja044964s.

DOI:10.1021/ja044964s

PMID:15701004

Abstract

Synergic stabilization of DNA triplexes by oligo-N3'-->P5' phosphoramidate (PN) modification and additions of comb-type cationic copolymers was demonstrated. The combination of the copolymer and the PN modification increased triplex K(a) about 4 orders of magnitude. Kinetic analysis revealed that observed stabilization resulted from kinetic complimentarity between increased association rates by the copolymer and decreased dissociation rates by the PN modification of triplex forming oligonucleotides. No countering interference between these stabilizing effects was observed. We propose that kinetic analyses of stabilizing effects permit selection of a rational combination of stabilizing methods for successful synergy in stabilizing complex formation.

摘要

寡聚 N3'→P5' 氨基磷酸酯（PN）修饰与梳型阳离子共聚物的添加对 DNA 三链体具有协同稳定作用。共聚物与 PN 修饰相结合使三链体的结合常数（K(a)）提高了约 4 个数量级。动力学分析表明，观察到的稳定性源于共聚物提高缔合速率与三链体形成寡核苷酸的 PN 修饰降低解离速率之间的动力学互补性。未观察到这些稳定作用之间的相互干扰。我们认为，对稳定作用进行动力学分析有助于选择合理的稳定方法组合，以在稳定复合物形成过程中成功实现协同作用。