大鼠小肠同种异体移植中的P物质和血管活性肠肽

Substance P and vasoactive intestinal peptide in rat small-bowel isografts.

作者信息

Tomita Ryouichi, Fujisaki Shigeru, Park Eichi, Ikeda Taro, Koshinaga Tugumichi

机构信息

Department of Surgery, Nippon Dental University School of Dentistry at Tokyo and Nippon Dental University Tokyo Hospital, 2-3-16 Fujimi, Chiyoda-ku, Tokyo 102-8158, Japan.

出版信息

Am J Surg. 2005 Jan;189(1):63-70. doi: 10.1016/j.amjsurg.2004.03.012.

DOI:10.1016/j.amjsurg.2004.03.012

PMID:15701495

Abstract

BACKGROUND

It is established that substance P (SP) is released by stimulation of nonadrenergic noncholinergic (NANC) excitatory nerves and vasoactive intestinal peptide (VIP) by stimulation of NANC inhibitory nerves. To evaluate the function of peptidergic nerves such as SP and VIP in small-bowel isografts, we examined the enteric nerve responses to SP and VIP in the isografted rat jejunum, using the normal rat jejunum as a control.

METHODS

Orthotopic entire small bowel transplantation (SBT) with portocaval drainage was performed from Lewis rats to Lewis rats. Grafted tissue specimens were obtained 130 days after SBT (n = 9). As controls, normal segments of the jejunum were obtained from untransplanted Lewis rats (n = 22). A mechanograph was used to evaluate in vitro jejunal responses to electrical field stimulation of the enteric nervous system before and after treatments with various autonomic nerve blockers and neuropeptides (SP and VIP).

RESULTS

SP concentration-dependently mediated the contraction reaction of NANC excitatory nerve in the isografted jejunum and to a lesser extent in the normal jejunum. In addition, there were significant diferences in the percentages showing contraction at 1 x 10(-8) and 1 x 10(-6)g/mL SP between the normal and isografted jejunal muscle strips (P < .05, respectively). VIP concentration dependently mediated the relaxation reaction of NANC inhibitory nerve in the normal jejunum and to a lesser extent in the isografted jejunum. In addition, there was a significant difference between the relaxation frequencies of the normal and those of isografted jejunal muscle strips at 1 x 10(-6) g/mL SP (P < .01).

CONCLUSIONS

Contraction reactions of SP were observed in both the normal and isografted jejunum but were increased in the isografted jejunum. Relaxation reactions of VIP were also observed in both the normal and isografted jejunum but were decreased in the isografted jejunum. The increase of the effects of SP via NANC excitatory nerves and the decrease of the effects of VIP in mediating NANC inhibitory nerves may be largely related to the peristaltic abnormalities seen in the isografted LEW rat jejunum.

摘要

背景

已证实P物质（SP）通过刺激非肾上腺素能非胆碱能（NANC）兴奋性神经释放，血管活性肠肽（VIP）通过刺激NANC抑制性神经释放。为评估SP和VIP等肽能神经在小肠同种异体移植中的功能，我们以正常大鼠空肠为对照，检测了同种异体移植大鼠空肠对SP和VIP的肠神经反应。

方法

将Lewis大鼠的小肠进行原位全小肠移植（SBT）并采用门腔分流术，受体为Lewis大鼠。小肠移植术后130天获取移植组织标本（n = 9）。作为对照，从未移植的Lewis大鼠获取正常空肠段（n = 22）。使用肌动描记器评估在使用各种自主神经阻滞剂和神经肽（SP和VIP）处理前后，空肠对肠神经系统电场刺激的体外反应。

结果

SP浓度依赖性地介导同种异体移植空肠中NANC兴奋性神经的收缩反应，在正常空肠中介导作用较小。此外，正常和同种异体移植空肠肌条在1×10⁻⁸和1×10⁻⁶g/mL SP时出现收缩的百分比存在显著差异（分别为P <.05）。VIP浓度依赖性地介导正常空肠中NANC抑制性神经的舒张反应，在同种异体移植空肠中介导作用较小。此外，正常和同种异体移植空肠肌条在1×10⁻⁶g/mL SP时的舒张频率存在显著差异（P <.01）。

结论

正常和同种异体移植空肠中均观察到SP的收缩反应，但同种异体移植空肠中的反应增强。正常和同种异体移植空肠中也均观察到VIP的舒张反应，但同种异体移植空肠中的反应减弱。通过NANC兴奋性神经SP作用的增强以及VIP介导NANC抑制性神经作用的减弱可能与同种异体移植LEW大鼠空肠中出现的蠕动异常密切相关。