基于凝集素结合特性对人体钩虫幼虫进行鉴别。

Distinction of human hookworm larvae based on lectin-binding characteristics.

作者信息

Kumar S, Pritchard D I

机构信息

Department of Life Sciences, University of Nottingham, University Park, UK.

出版信息

Parasite Immunol. 1992 Mar;14(2):233-7. doi: 10.1111/j.1365-3024.1992.tb00464.x.

DOI:10.1111/j.1365-3024.1992.tb00464.x

PMID:1570175

Abstract

A comparative study of lectin binding to ensheathed (EnL3) and exsheathed (ExL3) L3 larvae of Necator americanus, Ancylostoma duodenale and Ancylostoma ceylanicum revealed a number of differences between these hookworm species. These differences could provide a novel approach to distinguish infective L3 larvae in field conditions. For example, binding of Ulex europaeus agglutinin (UEA) and Ricinus communis agglutinin (RCA120) to N. americanus EnL3 distinguished them from those of A. duodenale and A. ceylanicum. Furthermore, UEA and RCA120 negative EnL3 could be separated into the two Ancylostoma sps. tested, as Dolichos biflorus agglutinin and Soybean agglutinin bound to EnL3 of A. ceylanicum but not to those of A. duodenale.

摘要

一项关于凝集素与美洲板口线虫、十二指肠钩口线虫和锡兰钩口线虫的被鞘L3幼虫（EnL3）及脱鞘L3幼虫（ExL3）结合情况的比较研究揭示了这些钩虫物种之间的一些差异。这些差异可为在野外条件下区分感染性L3幼虫提供一种新方法。例如，欧洲荆豆凝集素（UEA）和蓖麻凝集素（RCA120）与美洲板口线虫EnL3的结合使其与十二指肠钩口线虫和锡兰钩口线虫的EnL3区分开来。此外，UEA和RCA120阴性的EnL3可被分为所检测的两种钩口线虫属物种，因为双花扁豆凝集素和大豆凝集素与锡兰钩口线虫的EnL3结合，但不与十二指肠钩口线虫的EnL3结合。