水稻Na+/H+逆向转运蛋白Nhx1部分弥补了缺乏三种钠转运蛋白的酵母菌株对碱金属阳离子的敏感性。

Rice Na+/H+-antiporter Nhx1 partially complements the alkali-metal-cation sensitivity of yeast strains lacking three sodium transporters.

作者信息

Kinclová-Zimmermannová O, Flegelová H, Sychrová H

机构信息

Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czechia.

出版信息

Folia Microbiol (Praha). 2004;49(5):519-25. doi: 10.1007/BF02931527.

DOI:10.1007/BF02931527

PMID:15702539

Abstract

A triple mutant strain of Saccharomyces cerevisiae lacking its own Na+-ATPases and Na+/H+ antiporters (enal-4delta nha1delta nhx1delta) was used for the expression of the Oryza sativa NHX1 gene encoding a putative vacuolar Na+/H+ exchanger. Upon expression in yeast cells, the OsNhx 1p is not a transport system specific only for sodium cations but it has a broad substrate specificity for at least four alkali metal cations (Na+, Li+, K+ and Rb+) and is able to substitute for the endogenous yeast ScNhx1 antiporter. Its activity contributes to sequestration of alkali metal cations in intracellular vesicles.

摘要

使用一种缺乏自身钠-ATP酶和钠/氢反向转运蛋白的酿酒酵母三重突变株（enal-4δnha1δnhx1δ）来表达编码假定液泡钠/氢交换体的水稻NHX1基因。在酵母细胞中表达后，OsNhx 1p不是仅对钠阳离子具有特异性的转运系统，而是对至少四种碱金属阳离子（Na+、Li+、K+和Rb+）具有广泛的底物特异性，并且能够替代内源性酵母ScNhx1反向转运蛋白。其活性有助于将碱金属阳离子隔离在细胞内囊泡中。