利用流式细胞术对小林姬鼠和小家鼠肠道细菌进行快速革兰氏阳性菌计数及亚群评估。
Rapid G+ count and subpopulation assessment of the intestinal bacteria in Apodemus sylvaticus and Mus musculus by flow cytometry.
作者信息
Moragues M, Comas-Riu J, Vives-Rego J
机构信息
Departament de Microbiologia, Universitat de Barcelona, 080 28 Barcelona, Spain.
出版信息
Folia Microbiol (Praha). 2004;49(5):587-90. doi: 10.1007/BF02931538.
PMID:15702550
Abstract
We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial population and to describe bacterial subpopulations in the intestine.
摘要
我们报告了钙黄绿素乙酰甲酯在流式细胞术中的一种新应用，用于快速估计啮齿动物粪便（森林姬鼠和小家鼠）中革兰氏阳性菌的数量。我们还使用流式细胞术与Syto-13或Sypro Orange染色的联合应用来快速计数肠道中的细菌总数并描述细菌亚群。
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