核酸染料SYTO-13、TOTO-1和YOYO-1在通过流式细胞术研究大肠杆菌和海洋原核生物群体中的应用。
Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry.
作者信息
Guindulain T, Comas J, Vives-Rego J
机构信息
Departament de Microbiologia, Universitat de Barcelona, Spain.
出版信息
Appl Environ Microbiol. 1997 Nov;63(11):4608-11. doi: 10.1128/aem.63.11.4608-4611.1997.
Abstract
Three nucleic acid dyes (SYTO-13, TOTO-1, and YOYO-1) were tested on cultures of Escherichia coli and marine prokaryote populations. These dyes stain the RNA and DNA in E. coli but only respond to DNA in marine populations, according to the histograms obtained after DNase and RNase treatments.
摘要
对三种核酸染料（SYTO-13、TOTO-1和YOYO-1）进行了测试，测试对象为大肠杆菌培养物和海洋原核生物群体。根据经脱氧核糖核酸酶（DNase）和核糖核酸酶（RNase）处理后得到的直方图，这些染料可对大肠杆菌中的核糖核酸（RNA）和脱氧核糖核酸（DNA）进行染色，但仅对海洋群体中的DNA有反应。
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