捷克马疱疹病毒1型的分离及部分特性研究

Isolation and partial characterization of equine herpesvirus type 1 in Czechia.

作者信息

Molinková D, Celer V, Jahn P

机构信息

Institute of Microbiology and Immunology, Veterinary and Pharmaceutical University, 612 42 Brno, Czechia.

出版信息

Folia Microbiol (Praha). 2004;49(5):605-11. doi: 10.1007/BF02931542.

DOI:10.1007/BF02931542

PMID:15702554

Abstract

Equine herpesvirus type 1 was determined as the etiological cause of an abortion storm in Czechia in 2003 after the virus strain was isolated from aborted fetus and identified by serological means and by PCR technique. Cloning and sequencing of the glycoprotein D confirmed the identity of the isolates and showed molecular relationships to known EHV-1 strains. Comparison of glycoprotein D sequences with corresponding sequence of EHV-1 reference strains (Kentucky-A and Ab1) revealed high nucleotide homology. The Czech isolate of EHV-1 virus does not differ significantly from the Ab1 strain regarding the glycoprotein D gene and does not bear the frameshift in the 3' terminus which occurs in the Kentucky-A strain.

摘要

2003年，从流产胎儿中分离出病毒株，并通过血清学方法和聚合酶链反应（PCR）技术进行鉴定后，1型马疱疹病毒被确定为捷克一场流产风暴的病因。糖蛋白D的克隆和测序证实了分离株的身份，并显示了与已知EHV-1毒株的分子关系。将糖蛋白D序列与EHV-1参考毒株（肯塔基-A和Ab1）的相应序列进行比较，发现核苷酸同源性很高。就糖蛋白D基因而言，EHV-1病毒的捷克分离株与Ab1毒株没有显著差异，并且在3'末端没有肯塔基-A毒株中出现的移码突变。