Fuentealba Nadia A, Sguazza Guillermo H, Eöry Matías L, Valera Alejandro R, Pecoraro Marcelo R, Galosi Cecilia M
Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata.
Rev Argent Microbiol. 2011 Oct-Dec;43(4):273-7. doi: 10.1590/S0325-75412011000400007.
Equid herpesvirus 1 (EHV-1) infection has a significant economic impact on equine production, causing abortion, respiratory disease, neonatal death and neurological disorders. The identification of specific EHV-1 genes related to virulence and pathogenicity has been the aim of several research groups. The purpose of the present study was to analyze different genomic regions of Argentinean EHV-1 strains and to determine their possible relationship with virulence or clinical signs. Twenty-five EHV-1 Argentinean isolates recovered from different clinical cases between 1979 and 2007 and two reference strains were amplified and sequenced. The sequence alignments were carried out using Clustal X version 1.92 and the putative amino acid sequences were deduced using Bio-Edit version 7.05. Minor changes were observed. No changes that could be involved in the different virulence in the mouse model of three EHV-1 Argentinean strains were found. No genetic variants were observed. The genomic regions analyzed are unsuitable for differentiation between abortigenic strains and those isolated from neonatal deaths.
马疱疹病毒1型（EHV-1）感染对养马业具有重大经济影响，可导致流产、呼吸道疾病、新生驹死亡和神经紊乱。确定与毒力和致病性相关的特定EHV-1基因一直是多个研究小组的目标。本研究的目的是分析阿根廷EHV-1毒株的不同基因组区域，并确定它们与毒力或临床症状之间的可能关系。对1979年至2007年间从不同临床病例中分离出的25株阿根廷EHV-1毒株以及两株参考毒株进行了扩增和测序。使用Clustal X 1.92版本进行序列比对，并使用Bio-Edit 7.05版本推导推定的氨基酸序列。观察到微小变化。在三种阿根廷EHV-1毒株的小鼠模型中，未发现可能与不同毒力有关的变化。未观察到基因变异。所分析的基因组区域不适用于区分致流产毒株和从新生驹死亡病例中分离出的毒株。
