Liu Ning, Ying Cen, Li Kai, Liu Xiaoxue
Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu Sichuan 610041, P R China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Jan;19(1):42-4.
To explore the effect of nandrolone phenylpropionate (NP) on rats' fibroblasts after injury.
After being isolated from granulation tissue of a one-month Wistar rat,the fibroblasts(FB) were grouped into 5 groups and cultured in RPMI1640 and 5% FBS culturing liquid with 0.5, 1.0, 5.0, 10.0, and 15.0 microg/ml NP respectively. The control group was cultured in RPMI1640 and 5% FBS culturing liquid Fibroblasts were isolated from granulation tissue of a one-month Wistar rat and cultured with RPMI1640 culturing liquid with 5% FBS added different doses of NP from 0.5 approximately 15.0 microg/ml in NP group, but only 5% FBS for the control group. Cell validity of fibroblasts was measured by MTT. The proliferative index (PI) of the most effected group was measured by flow cytometry.
Compared with control group, higher FB validity occurred in every NP group (P < 0.05) The PI of FB in every NP group measured by flow cytometry was significantly higher than that in control group (P < 0.01).
NP can promote the replication and proliferation of FB.
探讨苯丙酸诺龙（NP）对大鼠损伤后成纤维细胞的影响。
从1月龄Wistar大鼠肉芽组织中分离出成纤维细胞（FB），分为5组，分别在含0.5、1.0、5.0、10.0和15.0μg/ml NP的RPMI1640和5%胎牛血清培养液中培养。对照组在RPMI1640和5%胎牛血清培养液中培养。从1月龄Wistar大鼠肉芽组织中分离成纤维细胞，NP组用添加不同剂量NP（0.5～15.0μg/ml）的含5%胎牛血清的RPMI1640培养液培养，对照组仅用5%胎牛血清培养。采用MTT法检测成纤维细胞的活性。用流式细胞术检测作用最明显组的增殖指数（PI）。
与对照组相比，各NP组FB活性均升高（P<0.05）。流式细胞术检测各NP组FB的PI均显著高于对照组（P<0.01）。
NP可促进FB的复制和增殖。
