Cole N A, Clark R N, Todd R W, Richardson C R, Gueye A, Greene L W, McBride K
ARS, USDA, Conservation and Production Research Laboratory, Bushland, TX 79012, USA.
J Anim Sci. 2005 Mar;83(3):722-31. doi: 10.2527/2005.833722x.
Emissions of ammonia, as well as other gases and particulates, to the atmosphere are a growing concern of livestock producers, the general public, and regulators. The concentration and ruminal degradability of CP in beef cattle diets may affect urinary and fecal excretion of N and thus may affect ammonia emissions from beef cattle feed yards. To determine the effects of dietary CP concentration and degradability on potential ammonia emissions, 54 steers were randomly assigned to nine dietary treatments in a 3 x 3 factorial arrangement of treatments. Treatments consisted of three dietary CP concentrations (11.5, 13, and 14.5%) and three supplemental urea:cottonseed meal ratios (100:0, 50:50, and 0:100 of supplemental N). Steers were confined to tie stalls, and feces and urine excreted were collected and frozen after approximately 30, 75, and 120 d on feed. One percent of daily urine and feces excretion were added to polyethylene chambers containing 1,550 g of soil. Chambers were sealed, and ammonia emissions were trapped in an acid solution for 7 d using a vacuum system. As the protein concentration in the diet increased from 11.5 to 13%, in vitro daily ammonia emissions increased (P < 0.01) 60 to 200%, due primarily to increased urinary N excretion. As days on feed increased, in vitro ammonia emissions also increased (P < 0.01). Potential ammonia losses were highly correlated (P < 0.01) to urinary N (r2 = 0.69), urinary urea-N (r2 = 0.58) excretion, serum urea-N concentration (r2 = 0.52), and intake of degradable protein N (r2 = 0.23). Although dietary composition can affect daily ammonia losses, daily ammonia emissions must be balanced with effects on animal performance to determine optimal protein concentrations and forms in the diet.
氨气以及其他气体和颗粒物向大气中的排放,日益引起畜牧生产者、普通公众和监管机构的关注。肉牛日粮中粗蛋白(CP)的浓度和瘤胃降解率可能会影响氮的尿液和粪便排泄,进而可能影响肉牛饲养场的氨气排放。为了确定日粮CP浓度和降解率对潜在氨气排放的影响,54头阉牛被随机分配到9种日粮处理组,采用3×3析因设计。处理包括三种日粮CP浓度(11.5%、13%和14.5%)和三种补充尿素与棉籽粕的比例(补充氮的比例为100:0、50:50和0:100)。阉牛被限制在拴系栏中,在采食约30、75和120天后,收集排出的粪便和尿液并冷冻。将每日尿液和粪便排泄量的1%添加到装有1550克土壤的聚乙烯室中。密封室,使用真空系统将氨气排放物收集在酸性溶液中7天。随着日粮中蛋白质浓度从11.5%增加到13%,体外每日氨气排放量增加(P<0.01)60%至200%,这主要是由于尿液氮排泄增加。随着采食天数增加,体外氨气排放量也增加(P<0.01)。潜在氨气损失与尿液氮(r2 = 0.69)、尿液尿素氮(r2 = 0.58)排泄、血清尿素氮浓度(r2 = 0.52)和可降解蛋白氮摄入量(r2 = 0.23)高度相关(P<0.01)。虽然日粮组成会影响每日氨气损失,但每日氨气排放必须与对动物生产性能的影响相平衡,以确定日粮中最佳的蛋白质浓度和形式。