瘤胃保护蛋氨酸——低蛋白日粮的一种饲料添加剂：对微生物种群、气体产生及发酵特性的影响
Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics.
作者信息
Abbasi Imtiaz Hussain Raja, Abbasi Farzana, Liu Lihui, Bodinga Bello M, Abdel-Latif Mervat A, Swelum Ayman A, Mohamed Mohamed Abdalla Elsiddig, Cao Yangchun
机构信息
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Department of Animal Nutrition, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Punjab, Pakistan.
出版信息
AMB Express. 2019 Jun 26;9(1):93. doi: 10.1186/s13568-019-0815-4.
The present study was performed to evaluate the effects of different concentration of rumen-protected methionine (RPMet) with a low level of crude protein (CP) using rumen simulation technology on many parameters. The experiment was assigned randomly into four treatments: (1) high protein diet (163.39 g/kg CP) without RPMet (HP); (2) low protein diet (146.33 g/kg CP) without RPMet (LP); (3) low protein diet, supplement with low RPMet (RPMet: 0.11 g/kg) (LPLMet); and (4) low protein diet, supplement with high RPMet (RPMet: 0.81 g/kg) (LPHMet), mixed with 20 g basal diet in each fermenter. Based on National Research Council (NRC) (Nutrient requirements of dairy cattle, National Academies Press, Washington, DC, 2001) recommendation for dairy ruminants HP diet was formulated as positive normal control and LP as a negative control. Results demonstrated that CP disappearance was found significantly higher (P < 0.05) in supplement groups compared with HP and found similar (P > 0.05) with LP. However, neutral detergent fiber (NDF) and gross energy (GE) were found a parallel among supplement groups compared to HP and higher than LP. Furthermore, microbial crude protein, total and short chain fatty acids were found similar in LPHMet and HP and found significantly higher than LPLMet and LP. The R. albus population of LPHMet found parallel to HP and pointedly higher than LP in a solid and liquid fraction. Daily production of ammonia nitrogen, total gas, and methane were higher in HP than LP, LPLMet, and LPHMet. Overall, results concluded that values of digestibility, rumen fermentation, microbial crude protein, and R. albus population were similar of LPHMet to that of HP group. However, production of ammonia-N, total gas, and methane volume were significantly higher in the HP group than LPLMet, LPHMet, and LP groups. In conclusion, rumen-protected methionine is a good feed supplement to low dietary protein in the level of 0.81 g/kg.
本研究旨在利用瘤胃模拟技术评估不同浓度的瘤胃保护性蛋氨酸（RPMet）对低粗蛋白（CP）水平下多个参数的影响。实验随机分为四个处理组：（1）不添加RPMet的高蛋白日粮（163.39 g/kg CP）（HP）；（2）不添加RPMet的低蛋白日粮（146.33 g/kg CP）（LP）；（3）低蛋白日粮，添加低水平RPMet（RPMet：0.11 g/kg）（LPLMet）；（4）低蛋白日粮，添加高水平RPMet（RPMet：0.81 g/kg）（LPHMet），每个发酵罐中均与20 g基础日粮混合。根据美国国家研究委员会（NRC）（《奶牛营养需求》，美国国家科学院出版社，华盛顿特区，2001年）对反刍动物日粮的建议，将HP日粮作为正对照，LP日粮作为负对照。结果表明，与HP组相比，添加组的CP消失率显著更高（P < 0.05），与LP组相似（P > 0.05）。然而，与HP组相比，添加组的中性洗涤纤维（NDF）和总能（GE）水平相当，且高于LP组。此外，LPHMet组和HP组的微生物粗蛋白、总短链脂肪酸和短链脂肪酸含量相似，显著高于LPLMet组和LP组。LPHMet组的白色瘤胃球菌数量在固相和液相部分与HP组相当，且明显高于LP组。HP组的氨氮、总气体和甲烷日产量高于LP组、LPLMet组和LPHMet组。总体而言，结果表明LPHMet组的消化率、瘤胃发酵、微生物粗蛋白和白色瘤胃球菌数量与HP组相似。然而，HP组的氨氮产量、总气体产量和甲烷产量显著高于LPLMet组、LPHMet组和LP组。总之，瘤胃保护性蛋氨酸是日粮蛋白水平为0.81 g/kg时的良好饲料添加剂。
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