Bai Hai-bo, Wang Jian-fei, Long Juan
Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
Zhongguo Zhong Yao Za Zhi. 2004 Mar;29(3):219-21.
To optimize the extraction process of root of Polygoni multflori and validate the interrelation between antioxdative capacity and the content of 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside in root of P. multiflori.
The optimum extraction was abserved with the orthogonal design; 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside was determined by HPLC and the antioxidative capacity by using photochemiluminescence detection method with the Photochem supplied by Analytik Jena AG; the concentration of ethanol, amount of ethanol, extraction time and extraction times were the four factors in the extraction.
The concentration of ethanol and extraction times had significant effect on the content of 2,3,4,5-tetrahydroxystilbene-2-O-beta-D-glucoside and the antioxidative capacity of the crude extract.
The best extraction process is to extract three times by using 10 fold EtOH(60%), refluxing at 85 degrees C and to extract one and half hours each time.
优化何首乌根的提取工艺，并验证何首乌根抗氧化能力与2,3,4,5-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量之间的相互关系。
采用正交设计观察最佳提取工艺；采用高效液相色谱法测定2,3,4,5-四羟基二苯乙烯-2-O-β-D-葡萄糖苷，使用德国耶拿分析仪器股份公司提供的光化学发光检测仪通过光化学发光检测法测定抗氧化能力；乙醇浓度、乙醇用量、提取时间和提取次数为提取过程中的四个因素。
乙醇浓度和提取次数对粗提取物中2,3,4,5-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量和抗氧化能力有显著影响。
最佳提取工艺为用10倍量60%乙醇，85℃回流，每次提取1.5小时，提取3次。
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