Enright Michael R, Griffin Christine T
Department of Biology and Institute of Bioengineering and Agroecology, National University of Ireland, Maynooth, Co. Kildare, Ireland.
J Invertebr Pathol. 2005 Jan;88(1):40-8. doi: 10.1016/j.jip.2004.10.002. Epub 2004 Nov 30.
The insect parasitic nematodes Heterorhabditis spp. are mutualistically associated with entomopathogenic bacteria, Photorhabdus spp. A novel association has been detected between H. megidis isolate EU17 and the endospore-forming bacterium Paenibacillus nematophilus. P. nematophilus sporangia adhere to infective juveniles (IJs) of H. megidis and develop in insect hosts along with the nematodes and their symbiont. We tested the effects of P. nematophilus on H. megidis. The yield and quality (size, energy reserves, and storage survival) of IJs were not affected by co-culture in insects with P. nematophilus. Dispersal of IJs in sand and on agar was inhibited by adhering P. nematophilus sporangia: fewer than 2% of IJs with P. nematophilus sporangia reached the bottom of a sand column, compared to 30% of the control treatment. Sporangia significantly reduced infectivity of H. megidis for wax moth larvae in sand, but not in a close contact (filter paper) assay. The results suggest that P. nematophilus may reduce the transmission potential of H. megidis through impeding the motility of IJs.
昆虫寄生线虫异小杆线虫属（Heterorhabditis spp.）与昆虫病原细菌发光杆菌属（Photorhabdus spp.）存在互利共生关系。在异小杆线虫（Heterorhabditis megidis）分离株EU17与产芽孢细菌嗜线虫芽孢杆菌（Paenibacillus nematophilus）之间发现了一种新的关联。嗜线虫芽孢杆菌的孢子囊附着在异小杆线虫的感染性幼虫（IJs）上，并与线虫及其共生菌一起在昆虫宿主体内发育。我们测试了嗜线虫芽孢杆菌对异小杆线虫的影响。在昆虫体内与嗜线虫芽孢杆菌共培养时，异小杆线虫感染性幼虫的产量和质量（大小、能量储备和储存存活率）不受影响。附着的嗜线虫芽孢杆菌孢子囊抑制了异小杆线虫感染性幼虫在沙子和琼脂上的扩散：附着有嗜线虫芽孢杆菌孢子囊的感染性幼虫中，不到2%到达沙柱底部，而对照处理的这一比例为30%。孢子囊显著降低了异小杆线虫在沙子中对蜡螟幼虫的感染力，但在紧密接触（滤纸）试验中则没有。结果表明，嗜线虫芽孢杆菌可能通过阻碍感染性幼虫的运动性来降低异小杆线虫的传播潜力。
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