含有猴免疫缺陷病毒nef基因的嵌合型人类免疫缺陷病毒1型病毒粒子对环孢菌素A具有抗性。
Chimeric human immunodeficiency virus type 1 virions that contain the simian immunodeficiency virus nef gene are cyclosporin A resistant.
作者信息
Khan Mahfuz, Jin Lingling, Miles Lesa, Bond Vincent C, Powell Michael D
机构信息
Dept. of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, 720 Westview Dr. SW, Atlanta, GA 30310, USA.
出版信息
J Virol. 2005 Mar;79(5):3211-6. doi: 10.1128/JVI.79.5.3211-3216.2005.
Abstract
We have previously shown that human immunodeficiency virus type 1 (HIV-1) virions which have their own nef gene deleted and are trans complemented to contain HIV-2 or simian immunodeficiency virus (SIV) Nef become resistant to treatment with cyclosporin A. To expand and confirm these studies, we have tested an HIV-1 isolate in which the HIV-1 nef gene has been replaced by the nef gene from SIV in a multiround infectivity assay using more physiologically relevant cell types. Our results confirm that HIV-1 virions that contain SIV nef can replicate in a cyclophilin-independent fashion.
摘要
我们之前已经表明，人类免疫缺陷病毒1型（HIV-1）病毒粒子若自身的nef基因缺失，并通过反式互补使其含有HIV-2或猴免疫缺陷病毒（SIV）的Nef，则会对环孢菌素A治疗产生抗性。为了扩展并确认这些研究，我们在一轮多轮感染性试验中使用了更具生理相关性的细胞类型，对一种HIV-1分离株进行了测试，该分离株中的HIV-1 nef基因已被SIV的nef基因所取代。我们的结果证实，含有SIV nef的HIV-1病毒粒子能够以不依赖亲环素的方式进行复制。
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Disruption of the actin cytoskeleton can complement the ability of Nef to enhance human immunodeficiency virus type 1 infectivity.
J Virol. 2004 Jun;78(11):5745-55. doi: 10.1128/JVI.78.11.5745-5755.2004.
2
Chimeric human immunodeficiency virus type 1 (HIV-1) virions containing HIV-2 or simian immunodeficiency virus Nef are resistant to cyclosporine treatment.
J Virol. 2004 Feb;78(4):1843-50. doi: 10.1128/jvi.78.4.1843-1850.2004.
3
HIV-1 Nef triggers Vav-mediated signaling pathway leading to functional and morphological differentiation of dendritic cells.
FASEB J. 2003 Nov;17(14):2025-36. doi: 10.1096/fj.03-0272com.
4
Cyclophilin A modulates the sensitivity of HIV-1 to host restriction factors.
Nat Med. 2003 Sep;9(9):1138-43. doi: 10.1038/nm910. Epub 2003 Aug 3.
5
Treatment of human immunodeficiency virus type 1 virions depleted of cyclophilin A by natural endogenous reverse transcription restores infectivity.
J Virol. 2003 Apr;77(7):4431-4. doi: 10.1128/jvi.77.7.4431-4434.2003.
6
Disassembly of human immunodeficiency virus type 1 cores in vitro reveals association of Nef with the subviral ribonucleoprotein complex.
J Virol. 2003 Apr;77(7):4409-14. doi: 10.1128/jvi.77.7.4409-4414.2003.
7
Properties of a chimeric simian-human immunodeficiency virus expressing an hybrid HIV-1 Nef/SIVmac Nef protein.
Arch Virol. 2002 Oct;147(10):1963-75. doi: 10.1007/s00705-002-0857-8.
8
An intracellular block to primate lentivirus replication.
Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11549-51. doi: 10.1073/pnas.192449399. Epub 2002 Aug 23.
9
Cyclophilin a plays distinct roles in human immunodeficiency virus type 1 entry and postentry events, as revealed by spinoculation.
J Virol. 2002 May;76(9):4671-7. doi: 10.1128/jvi.76.9.4671-4677.2002.
10
Restoration of wild-type infectivity to human immunodeficiency virus type 1 strains lacking nef by intravirion reverse transcription.
J Virol. 2001 Dec;75(24):12081-7. doi: 10.1128/JVI.75.24.12081-12087.2001.
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