Nakatsuji N, Løvtrup S
Arch Anat Microsc Morphol Exp. 1978;67(3):185-9.
A method based on the degradation by enzymes and nitrous acid of isotopically labelled glycosaminoglycans has been employed to study the synthesis of these compounds in normal, animalized and vegetalized sea urchin embryos. According to standard criteria, these organisms synthesize dermatan sulfate, heparan sulfate, hyaluronate and keratan sulfate. The hyaluronate seems to be slightly sulfated, it may thus be mucoitin sulfate. The preliminary results obtained suggest a conspicuous difference between animalized and vegetalized embryos: the synthesis of dermatan sulfate is suppressed in the former, while proceeding normally in the latter. The synthesis of heparan sulfate is not affected by our experimental conditions, but the isotope incorporation in hyaluronate and in keratan sulfate is decreased, more in the vegetalized than in the animalized embryos.
一种基于用酶和亚硝酸降解同位素标记的糖胺聚糖的方法已被用于研究这些化合物在正常、动物化和植物化海胆胚胎中的合成。根据标准标准，这些生物体合成硫酸皮肤素、硫酸乙酰肝素、透明质酸和硫酸角质素。透明质酸似乎有轻微硫酸化，因此可能是硫酸黏液素。获得的初步结果表明动物化胚胎和植物化胚胎之间存在显著差异：前者中硫酸皮肤素的合成受到抑制，而后者中则正常进行。硫酸乙酰肝素的合成不受我们实验条件的影响，但透明质酸和硫酸角质素中的同位素掺入减少，植物化胚胎中的减少程度比动物化胚胎中的更大。
