一种用于并行检测食品中五种转基因事件的微阵列平台：聚合酶链反应-连接检测反应-通用阵列联合方法

A microarray platform for parallel detection of five transgenic events in foods: a combined polymerase chain reaction-ligation detection reaction-universal array method.

作者信息

Bordoni Roberta, Germini Andrea, Mezzelani Alessandra, Marchelli Rosangela, De Bellis Gianluca

机构信息

Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, I-20090 Segrate (MI), Italy.

出版信息

J Agric Food Chem. 2005 Feb 23;53(4):912-8. doi: 10.1021/jf0486949.

DOI:10.1021/jf0486949

PMID:15712997

Abstract

We recently developed a multiplex polymerase chain reaction (PCR) system for the simultaneous detection of four transgenic maize (MON810, Bt176, Bt11, and GA21), one transgenic soybean (Roundup Ready), and two control genes (lectin and zein). Because PCR can lead to ambiguous interpretations due to low specificity, we have developed the ligation detection reaction (LDR) combined with a universal array as a molecular tool to confirm results of PCR analysis. Here, we describe the PCR-LDR-universal array procedure and demonstrate its specificity in revealing the presence of transgenic DNA in experimental samples, raw materials, and commercial foodstuffs.

摘要

我们最近开发了一种多重聚合酶链反应（PCR）系统，用于同时检测四种转基因玉米（MON810、Bt176、Bt11和GA21）、一种转基因大豆（抗草甘膦大豆）以及两个对照基因（凝集素和玉米醇溶蛋白）。由于PCR可能因特异性低而导致结果解读不明确，我们开发了连接检测反应（LDR）并结合通用阵列作为一种分子工具，以确认PCR分析的结果。在此，我们描述了PCR-LDR-通用阵列程序，并展示了其在揭示实验样品、原材料和商业食品中转基因DNA存在情况方面的特异性。