来氟米特和西多福韦对BK病毒在体外培养系统中复制的影响。
Effect of leflunomide and cidofovir on replication of BK virus in an in vitro culture system.
作者信息
Farasati Noush Afarin, Shapiro Ron, Vats Abhay, Randhawa Parmjeet
机构信息
Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA.
出版信息
Transplantation. 2005 Jan 15;79(1):116-8. doi: 10.1097/01.tp.0000149338.97084.5f.
BACKGROUND
Cidofovir and Leflunomide are used empirically in the treatment of BK virus nephropathy. The aim of this study is to quantify the antiviral activity of these drugs.
METHODS
BK virus was grown in a cell-culture system. The rate of viral replication in the presence or absence of the drug being tested was assessed using a quantitative polymerase chain reaction assay.
RESULTS
The inhibitory concentration, effective concentration, and selectivity index for Leflunomide are 39.7+/-6.9, 11.3+/-2.8, and 3.8+/-0.8 microg/mL, respectively. For Cidofovir, these indices were, respectively, 63.9+/-17.2, 36.3+/-11.7, and 2.3+/-0.8 microg/mL.
CONCLUSIONS
The in vitro activity of Cidofovir and Leflunomide is modest, and the selectivity index is low. There is a need to develop more effective and less toxic anti-BK virus drugs for clinical use.
背景
西多福韦和来氟米特经验性用于治疗BK病毒肾病。本研究的目的是量化这些药物的抗病毒活性。
方法
BK病毒在细胞培养系统中培养。使用定量聚合酶链反应测定法评估在有或没有受试药物存在的情况下病毒复制的速率。
结果
来氟米特的抑制浓度、有效浓度和选择性指数分别为39.7±6.9、11.3±2.8和3.8±0.8微克/毫升。对于西多福韦，这些指数分别为63.9±17.2、36.3±11.7和2.3±0.8微克/毫升。
结论
西多福韦和来氟米特的体外活性适中，且选择性指数较低。需要开发更有效且毒性更小的抗BK病毒药物用于临床。
Zotero 插件