Xu Jun-Feng, Jiang Ling, Wang Guo-Gang, Liu Shi-Jia, Chen Liang-Ming, Wang Chun-Ming, Luo Lin-Guang, Wan Jian-Min
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan Xue Bao. 2005 Jan;32(1):57-65.
PeiAi64S, a photoperiod and thermo-sensitive genic male sterile, has been wildly applied to hybrid rice seed production in China, but its photoperiod-sensitivity gene for heading date in this sterile line was still unknown. This definitely limited the further use of this sterile line in breeding practice and re-production of hybrid seeds. To solve this problem, using heading time nearly isogenic lines EGO - EG7, ER - LR, T65 - T65m and six heading date QTL-isogenic lines, NIL (Hd1) - NIL (Hd6) with the genes of Nipponbare but Hd1 - Hd6 genes from Kasalath respectively, we performed a genetic analysis of PeiAi64S with special reference to photoperiod-sensitivity loci, in natural long days at Nanjing (32 degrees N) where the average day-length is about 14 h and in natural short days at Linshui county, Hainan province(18 degrees 29'N), where the average day-length is about 11.6 h during the course of rice growing respectively. The F1 and F2 generations from the crosses 'PeiAi64S x heading time nearly isogenic lines' were subjected to genetic analyses. Experimental results showed that PeiAi64S carries photoperiod-sensitivity allele gene E1 and E3 and dominant earliness gene Ef-1 in E1 and E3 and Ef-1 loci, respectively, and a photoperiod insensitivity allele Se-1 degrees in Se-1 locus. Meanwhile, the photoperiod-sensitivity gene E1 and photoperiod-insensitivity gene Se-1e in PeiAi64S were also identified by crossing with the NIL(Hd1) and NIL(Hd4). In addition, a recessive inhibitor for photoperiod-sensitive gene E1 or Se-1(n) and other modified photoperiod-sensitive genes: i-Se-1, E3, Hd3 (En-Se-1), Hd5 and Hd6, were identified in PeiAi64S by crossing with QTL nearly isogenic lines: NIL(Hd2), NIL (Hd3), NIL (Hd5) and NIL( Hd6), The results indicated that the genotype of PeiAi64S's heading date was: E1E1e2e2E3E3Se-1(e)Se-1(e)Ef-1 Ef-1 i-Se-1 i-Se-1.
培矮64S是一种光温敏核不育系，已在中国杂交水稻制种中广泛应用，但其抽穗期光周期敏感基因仍不清楚。这无疑限制了该不育系在育种实践和杂交种子繁殖中的进一步应用。为解决这一问题，利用抽穗期近等基因系EGO - EG7、ER - LR、T65 - T65m以及6个抽穗期QTL近等基因系，即分别带有日本晴基因但Hd1 - Hd6基因来自卡萨拉思的NIL（Hd1） - NIL（Hd6），我们分别在南京（北纬32度）自然长日照条件下（水稻生长期间平均日长约14小时）和海南省临水县（北纬18度29分）自然短日照条件下（水稻生长期间平均日长约11.6小时），对培矮64S进行了遗传分析，特别关注光周期敏感位点。对“培矮64S×抽穗期近等基因系”杂交组合的F1和F2代进行了遗传分析。实验结果表明，培矮64S在E1、E3和Ef - 1位点分别携带光周期敏感等位基因E1和E3以及显性早熟基因Ef - 1，在Se - 1位点携带光周期不敏感等位基因Se - 1e。同时，通过与NIL（Hd1）和NIL（Hd4）杂交，还鉴定出了培矮64S中的光周期敏感基因E1和光周期不敏感基因Se - 1e。此外，通过与QTL近等基因系NIL（Hd2）、NIL（Hd3）、NIL（Hd）和NIL（Hd6）杂交，在培矮64S中鉴定出了光周期敏感基因E1或Se - 1的隐性抑制基因Se - 1（n）以及其他修饰光周期敏感基因：i - Se - 1、E3、Hd3（En - Se - 1）、Hd5和Hd6。结果表明，培矮64S抽穗期的基因型为：E1E1e2e2E3E3Se - 1（e）Se - 1（e）Ef - 1 Ef - 1 i - Se - 1 i - Se - 1。
