Li De-xin, Wang Si-ling, Ito Yoshimasa, Zhang Jian-hai, Wu Chun-fu
Department of Pharmaceutics, College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Acta Pharmacol Sin. 2005 Mar;26(3):359-63. doi: 10.1111/j.1745-7254.2005.00041.x.
To investigate the biological activity of human lens epithelial cells (HLEC) in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and to assesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels and expression of NOS.
The human lens epithelial cell line SRA 01/04 was used in this experiment. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect, respectively, iNOS mRNA expression and protein production.
A costimulation by interferon gamma (IFN-gamma) and lipopolysaccharide (LPS) was necessary for iNOS expression in HLEC. The expression of iNOS was significantly reduced in a dose-dependent manner by adding DDC from 10 micromol/L to 1 mmol/L.
The expression of iNOS in HLEC needs co-stimulation with IFN-gamma and LPS and it is inhibited by DDC.
研究人晶状体上皮细胞（HLEC）产生诱导型一氧化氮合酶（iNOS）和一氧化氮（NO）的生物学活性，并评估二乙基二硫代氨基甲酸盐（DDC）对iNOS mRNA水平和一氧化氮合酶（NOS）表达的影响。
本实验采用人晶状体上皮细胞系SRA 01/04。分别用半定量逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测iNOS mRNA表达和蛋白质生成。
γ干扰素（IFN-γ）和脂多糖（LPS）共同刺激是HLEC中iNOS表达所必需的。加入10微摩尔/升至1毫摩尔/升的DDC可使iNOS表达以剂量依赖性方式显著降低。
HLEC中iNOS的表达需要IFN-γ和LPS共同刺激，且受DDC抑制。
